05-777 | Anti-Phosphotyrosine Antibody, recombinant clone 4G10®

05-777
1 mg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-777
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10®
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: EGF stim A431 Cell Lysate (12-302).
      PresentationProtein G purified recombinant 4G10 mouse IgG2bk in 0.01M Phosphate Buffer containing no preservative, pH 7.1
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10 is a recombinant antibody that detects tyrosine phosphorylated proteins in all species. This unique recombinant antibody is validated for use in IC, IH, IP, WB and is published in peer review journals.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenProduced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via Nickel affinity matrices.
      Clone4G10®
      HostMouse
      SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
      IsotypeIgG2bκ
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      Molecular Weightvaries
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Phosphotyrosine, recombinant -2798545 2798545
      Anti-Phosphotyrosine, recombinant 4G10 (mouse monoclonal IgG2bk) 2869703
      Anti-Phosphotyrosine, recombinant 4G10 (mouse monoclonal IgG2bk) 2938435
      Anti-Phosphotyrosine, recombinant 4G10 (mouse monoclonal IgG2bk) 2909566
      Anti-Phosphotyrosine, recombinant 4G10 (mouse monoclonal IgG2bk) - DAM1460246 DAM1460246
      Anti-Phosphotyrosine, recombinant clone 4G10 - 2006565 2006565
      Anti-Phosphotyrosine, recombinant clone 4G10 ® - 2527460 2527460
      Anti-Phosphotyrosine, recombinant clone 4G10 ® -2506491 2506491
      Anti-Phosphotyrosine, recombinant clone 4G10#174; - DAM1498505 DAM1498505
      Anti-Phosphotyrosine, recombinant clone 4G10#174; - DAM1564514 DAM1564514

      References

      Reference overviewApplicationPub Med ID
      12/15-Lipoxygenase mediates high-fat diet-induced endothelial tight junction disruption and monocyte transmigration: a new role for 15(S)-hydroxyeicosatetraenoic acid in endothelial cell dysfunction.
      Kundumani-Sridharan, V; Dyukova, E; Hansen, DE; Rao, GN
      The Journal of biological chemistry  288  15830-42  2013

      Show Abstract
      23589307 23589307
      Both Kdr and Flt1 play a vital role in hypoxia-induced Src-PLD1-PKCγ-cPLA(2) activation and retinal neovascularization.
      Singh, NK; Hansen, DE; Kundumani-Sridharan, V; Rao, GN
      Blood  121  1911-23  2013

      Show Abstract
      23319572 23319572
      15-Lipoxygenase-1-enhanced Src-Janus kinase 2-signal transducer and activator of transcription 3 stimulation and monocyte chemoattractant protein-1 expression require redox-sensitive activation of epidermal growth factor receptor in vascular wall remodeling.
      Singh, NK; Wang, D; Kundumani-Sridharan, V; Van Quyen, D; Niu, J; Rao, GN
      The Journal of biological chemistry  286  22478-88  2011

      Show Abstract
      21536676 21536676
      Cytosolic lysine residues enhance anterograde transport and activation of the erythropoietin receptor.
      Liron Yosha,Orly Ravid,Nathalie Ben-Califa,Drorit Neumann
      The Biochemical journal  435  2011

      Show Abstract
      21291419 21291419
      Inhibition of Grb2 expression demonstrates an important role in BCR-ABL-mediated MAPK activation and transformation of primary human hematopoietic cells.
      Modi, H; Li, L; Chu, S; Rossi, J; Yee, JK; Bhatia, R
      Leukemia  25  305-12  2011

      Show Abstract
      Western Blotting21072043 21072043
      Motesanib inhibits Kit mutations associated with gastrointestinal stromal tumors.
      Caenepeel, S; Renshaw-Gegg, L; Baher, A; Bush, TL; Baron, W; Juan, T; Manoukian, R; Tasker, AS; Polverino, A; Hughes, PE
      Journal of experimental & clinical cancer research : CR  29  96  2010

      Show Abstract Full Text Article
      20633291 20633291
      Interplay between the heterotrimeric G-protein subunits Galphaq and Galphai2 sets the threshold for chemotaxis and TCR activation.
      Ngai, J; Inngjerdingen, M; Berge, T; Taskén, K
      BMC immunology  10  27  2009

      Show Abstract Full Text Article
      19426503 19426503

      FAQ

      QuestionAnswer
      Why can't I use this antibody with the Catch and Release® v2.0 Reversible Immunoprecipitation System?The Anti-Phosphotyrosine, recombinant clone 4G10® contains a hexa-histidine (6X-His) tag at the c-terminus. The Catch and Release® v2.0 Reversible Immunoprecipitation System is incompatible with any hexa-histidine tagged proteins. If you would like to use Anti-Phosphotyrosine, recombinant clone 4G10® and The Catch and Release® v2.0 Reversible Immunoprecipitation System, then you should immunoprecipitate first with Anti-Phosphotyrosine, clone 4G10® (catalog #05-321) and then you could use either the Anti-Phosphotyrosine, recombinant clone 4G10® or the Anti-Phosphotyrosine, clone 4G10® (catalog #05-321) for the western blot.
      What is the difference between Anti-Phosphotyrosine, recombinant clone 4G10® (catalog #05-777) and Anti-Phosphotyrosine, clone 4G10® (catalog #05-321)?Anti-Phosphotyrosine, recombinant clone 4G10® (catalog #05-777) and Anti-Phosphotyrosine, clone 4G10® (catalog #05-321) are similar in performance. If you are interested in using Anti-Phosphotyrosine over a long period of time, you may want to use the Anti-Phosphotyrosine, recombinant clone 4G10® (catalog #05-777) as lot-to-lot variability is essentially eliminated.

      Related Products & Applications

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      Included Positive Control

      Catalogue Number Description  
      12-302 EGF-Stimulated A431 Cell Lysate Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies