06-1299 | Anti-Pontin (Tip49) Antibody

06-1299
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, CaWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1299
      DescriptionAnti-Pontin (Tip49) Antibody
      Alternate Names
      • 49 kDa TATA box-binding protein-interacting protein
      • 49 kDa TBP-interacting protein
      • 54 kDa erythrocyte cytosolic protein
      • INO80 complex subunit H
      • NMP 238
      • Nuclear matrix protein 238
      • Pontin 52
      • RuvB (E coli homolog)-like 1
      • RuvB-like 1
      • RuvB-like 1 (E. coli)
      • TATA binding protein interacting protein 49 kDa
      • TIP60-associated protein 54-alpha
      Background InformationPontin/TIP49 (also known as Pontin 52, TIP49a, or 49 kDa TATA box-binding protein-interacting protein) is a member of the ruvB family. Members of this family are known to have ATP-dependent DNA helicase and a single-stranded, DNA-stimulated ATPase activities. Pontin participates in select transcriptional activation as a component of the NuA4 histone acetyltransferase complex that is thought to be necessary for the activation of specific transcription pathways related to oncogenesis. Pontin is a critical factor in cell proliferation and, when present on the cell surface, may bind to plasminogen and upregulate the activation of plasminogen. Expression of pontin is considered ubiquitous; however significantly high levels have been reported in skeletal muscle, testis, and heart tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Pontin (Tip49) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Pontin (Tip49) also known as 49 kDa TATA box-binding protein-interacting protein or Nuclear matrix protein 238.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pontin (TIP49) in NIH/3T3, HeLa, and A431 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human pontin.
      EpitopeRecognizes Pontin/Tip49 at a region near the amino terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Pontin/Tip49 at a region near the amino terminus
      Species Reactivity
      • Human
      • Mouse
      • Canine
      Species Reactivity NoteDemonstrated to react with human and mouse.
      Predicted to react with canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ECP-54
      • ECP54
      • INO80H
      • NMP238
      • PONTIN
      • Pontin52
      • RVB1
      • Rvb1
      • TAP54-alpha
      • TIH1
      • TIP49
      • TIP49A
      • TIP49a
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.

      May be able to bind plasminogen at cell surface and enhance plasminogen activation.

      Essential for cell proliferation.

      CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

      SUBUNIT STRUCTURE: Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Interacts with IGHMBP2. Interacts with OFD1.

      SUBCELULAR LOCATION: Nucleus matrix. Nucleus › nucleoplasm. Cytoplasm. Membrane. Cytoplasm › cytoskeleton › centrosome. Note: Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.

      TISSUE SPECIFICTY: Ubiquitously expressed with high expression in heart, skeletal muscle and testis.

      DOMAIN: Binding to MYC is dependent on a Myc domain essential for oncogenic activity.

      MISCELLANEOUS: High level of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.

      SEQUENCE SIMILARITIES: Belongs to the ruvB family.
      Molecular Weight ~52 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Pontin (Tip49) in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Pontin (Tip49) - 2397151 2397151
      Anti-Pontin (Tip49) - 2445055 2445055
      Anti-Pontin (Tip49) - 1967274 1967274
      Anti-Pontin (Tip49) - 2207242 2207242
      Anti-Pontin (Tip49) - NRG1782321 NRG1782321
      Anti-Pontin (Tip49) -2676584 2676584
      Anti-Pontin (Tip49) Polyclonal Antibody 2988817

      References

      Reference overviewPub Med ID
      The cytoplasmic tail of CD44 is required for basolateral localization in epithelial MDCK cells but does not mediate association with the detergent-insoluble cytoskeleton of fibroblasts.
      S J Neame,C M Isacke
      The Journal of cell biology  121  1993

      Show Abstract Full Text Article
      8509451 8509451