06-1058 | Anti-Protein Wnt-5a Antibody

06-1058
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Rb, ShWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1058
      DescriptionAnti-Protein Wnt-5a Antibody
      Alternate Names
      • wingless-type MMTV integration site family, member 5A
      • WNT-5A protein
      • protein Wnt-5a
      Background InformationThe Wnt family of cysteine-rich proteins constitutes a group of ligands known for their roles in development such as cell differentiation, proliferation, migration and embryonic patterning. They have also been implicated in the formation of tumors. Wnts are involved in the stimulation of several signaling cascades that have been divided into two types; the canonical pathway, and the non-canonical pathway. Protein Wnt5a is considered to be a non-canonical ligand for the Wnt signaling pathway and is a ligand to the frizzled family of seven transmembrane receptors. When interacting with ROR2, protein Wnt5a is considered to inhibit tumor formation due to protein Wnt5a’s suppression of the canonical Wnt signaling pathway.Protein Wnt5a is involved in activating cell migration, regulating the motility of melanoma cells, decreasing the proliferation, chondrogenesis, and migration of carcinoma cells, and inhibiting the expression of type II collagen in chondrocytes. Protein Wnt5a is a requirement to the endothelial differentiation and vascularization of Embryonic Stem Cells, and is also necessary to the development of mammary glands, limb outgrowth, and the genital tubercle.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human heart tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Protein Wnt-5a Antibody is validated for use in WB, IP for the detection of Protein Wnt-5a.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot (SNAP ID) Analysis: 5 µg/mL from a previous lot detected Protein Wnt-5a on 10 µg of human heart tissue lysate.

      Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Protein Wnt-5a from 500 µg of human adult normal heart RIPA lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Protein Wnt-5a.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rabbit
      • Sheep
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with rabbit and sheep based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].
      Gene Symbol
      • hWNT5A
      • WNT5A
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

      SUBUNIT STRUCTURE: Interacts with PORCN (By similarity). Interacts with WLS (By similarity).

      SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix.

      TISSUE SPECIFICITY: Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).

      PTM: Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding (By similarity).

      Glycosylation is necessary for secretion but not for activity (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the Wnt family.
      Molecular Weight~ 42 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human heart tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Protein Wnt-5a on 10 µg of human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies