06-559 | Anti-Pyk2 Antibody

06-559
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-559
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Pyk2 Antibody
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain cytosolic RIPA lysates
      PresentationProtein A Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationThis Anti-Pyk2 Antibody is validated for use in IC, IP, WB for the detection of Pyk2.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST-fusion protein containing amino acids 726-863 of human Pyk2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Pyk2, and its degradation products; does not cross-react with FAK.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
      Gene Symbol
      • PTK2B
      • RAFTK
      • FAK2
      • PYK2
      • FADK2
      • PTK
      • CADTK
      • CAKB
      • PKB
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14289 # Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
      SIZE: 1009 amino acids; 115875 Da
      SUBUNIT: Interacts with Crk-associated substrate (Cas), PTPNS1 and SH2D3C (By similarity). Interacts with nephrocystin, DDEF2, OPHN1L, SKAP2 and TGFB1I1.
      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interaction with nephrocystin induces the membrane-association of the kinase.
      TISSUE SPECIFICITY: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
      PTM: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha- lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity (By similarity).
      SIMILARITY: SwissProt: Q14289 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on mouse brain cytosolic RIPA lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Pyk2 (rabbit polyclonal IgG) 2884538
      Anti-Pyk2 - 2424764 2424764
      Anti-Pyk2 - 15306 15306
      Anti-Pyk2 - 17748 17748
      Anti-Pyk2 - 20648 20648
      Anti-Pyk2 - 2278504 2278504
      Anti-Pyk2 - 2510371 2510371
      Anti-Pyk2 - 26172 26172
      Anti-Pyk2 -2723611 2723611

      References

      Reference overviewApplicationPub Med ID
      HBZ stimulates brain-derived neurotrophic factor/TrkB autocrine/paracrine signaling to promote survival of human T-cell leukemia virus type 1-Infected T cells.
      Polakowski, N; Terol, M; Hoang, K; Nash, I; Laverdure, S; Gazon, H; Belrose, G; Mesnard, JM; Césaire, R; Péloponèse, JM; Lemasson, I
      Journal of virology  88  13482-94  2014

      Show Abstract
      25210182 25210182
      Collagen XV inhibits epithelial to mesenchymal transition in pancreatic adenocarcinoma cells.
      Clementz, AG; Mutolo, MJ; Leir, SH; Morris, KJ; Kucybala, K; Harris, H; Harris, A
      PloS one  8  e72250  2013

      Show Abstract
      23991074 23991074
      p21 as a transcriptional co-repressor of S-phase and mitotic control genes.
      Ferrándiz, N; Caraballo, JM; García-Gutierrez, L; Devgan, V; Rodriguez-Paredes, M; Lafita, MC; Bretones, G; Quintanilla, A; Muñoz-Alonso, MJ; Blanco, R; Reyes, JC; Agell, N; Delgado, MD; Dotto, GP; León, J
      PloS one  7  e37759  2012

      Show Abstract
      22662213 22662213
      Decreased cell adhesion promotes angiogenesis in a Pyk2-dependent manner.
      Shen CJ, Raghavan S, Xu Z, Baranski JD, Yu X, Wozniak MA, Miller JS, Gupta M, Buckbinder L, Chen CS.
      Experimental cell research  317  1860-71  2011

      Show Abstract
      21640103 21640103
      The miR-17-92 microRNA cluster is regulated by multiple mechanisms in B-cell malignancies.
      Ji, M; Rao, E; Ramachandrareddy, H; Shen, Y; Jiang, C; Chen, J; Hu, Y; Rizzino, A; Chan, WC; Fu, K; McKeithan, TW
      The American journal of pathology  179  1645-56  2011

      Show Abstract
      21806958 21806958
      Luminal alkalinization attenuates proteinuria-induced oxidative damage in proximal tubular cells.
      Souma, T; Abe, M; Moriguchi, T; Takai, J; Yanagisawa-Miyazawa, N; Shibata, E; Akiyama, Y; Toyohara, T; Suzuki, T; Tanemoto, M; Abe, T; Sato, H; Yamamoto, M; Ito, S
      Journal of the American Society of Nephrology : JASN  22  635-48  2011

      Show Abstract
      Western Blotting21372211 21372211
      Mad3 negatively regulates B cell differentiation in the spleen by inducing Id2 expression.
      Gore, Y; Lantner, F; Hart, G; Shachar, I
      Molecular biology of the cell  21  1864-71  2010

      Show Abstract Full Text Article
      20375148 20375148
      Expression of a protein involved in bone resorption, Dkk1, is activated by HTLV-1 bZIP factor through its activation domain.
      Polakowski, N; Gregory, H; Mesnard, JM; Lemasson, I
      Retrovirology  7  61  2010

      Show Abstract Full Text Article
      Western Blotting20653953 20653953
      Pyk2 inhibition of p53 as an adaptive and intrinsic mechanism facilitating cell proliferation and survival.
      Ssang-Taek Lim,Nichol L G Miller,Ju-Ock Nam,Xiao Lei Chen,Yangmi Lim,David D Schlaepfer
      The Journal of biological chemistry  285  2010

      Show Abstract Full Text Article
      19880522 19880522
      Epigenetic analysis of the critical region I for premature ovarian failure: demonstration of a highly heterochromatic domain on the long arm of the mammalian X chromosome.
      Rizzolio, F; Pramparo, T; Sala, C; Zuffardi, O; De Santis, L; Rabellotti, E; Calzi, F; Fusi, F; Bellazzi, R; Toniolo, D
      Journal of medical genetics  46  585-92  2009

      Show Abstract
      18628312 18628312

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      Title
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies