Key Spec Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H, R||IP, WB||M||Ascites||Monoclonal Antibody|
|Description||Anti-Pyk2/CAKβ Antibody, clone 74|
|Presentation||250 μl of mouse ascites containing 0.05% sodium azide. Frozen at -20°C.|
|Application||Anti-Pyk2/CAKβ Antibody, clone 74 is an antibody against Pyk2/CAKβ for use in IP & WB.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||2 years at -20°C|
|Material Size||250 µL|
|Anti-Pyk2/CAKB, clone 74 (mouse ascites)||2920716|
|Anti-Pyk2/CAKb, clone 74 (mouse ascites) - 2025015||2025015|
|Anti-Pyk2/CAKb, clone 74 (mouse ascites) - 2243476||2243476|
|Anti-Pyk2/CAKb, clone 74 - 2043517||2043517|
|Anti-Pyk2/CAKb, clone 74 - DAM1493398||DAM1493398|
|Anti-Pyk2/CAKbeta;, clone 74 - 17028||17028|
|Anti-Pyk2/CAKbeta;, clone 74 - 22940||22940|
|Anti-Pyk2/CAKbeta;, clone 74 - 31834||31834|
|Anti-Pyk2/CAKβ, clone 74 (mouse ascites)||2988096|
|Anti-Pyk2/CAKβ, clone 74 -2772638||2772638|
|Reference overview||Pub Med ID|
|Integrin signaling cascades are operational in adult hippocampal synapses and modulate NMDA receptor physiology.|
Joie A Bernard-Trifilo, Enikö A Kramár, Reidun Torp, Ching-Yi Lin, Eduardo A Pineda, Gary Lynch, Christine M Gall
Journal of neurochemistry 93 834-49 2005
Integrin class adhesion proteins are concentrated at adult brain synapses. Whether synaptic integrins engage kinase signaling cascades has not been determined, but is a question of importance to ideas about integrin involvement in functional synaptic plasticity. Accordingly, synaptoneurosomes from adult rat brain were used to test if matrix ligands activate integrin-associated tyrosine kinases, and if integrin signaling targets include NMDA-class glutamate neurotransmitter receptors. The integrin ligand peptide Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) induced rapid (within 5 min) and robust increases in tyrosine phosphorylation of focal adhesion kinase, proline-rich tyrosine kinase 2 and Src family kinases. Increases were similarly induced by the native ligand fibronectin, blocked with neutralizing antibodies to beta1 integrin, and not obtained with control peptides, indicating that kinase activation was integrin-mediated. Both GRGDSP and fibronectin caused rapid Src kinase-dependent increases in tyrosine phosphorylation of NMDA receptor subunits NR2A and NR2B in synaptoneurosomes and acute hippocampal slices. Tests of the physiological significance of the latter result showed that ligand treatment caused a rapid and beta1 integrin-dependent increase in NMDA receptor-mediated synaptic responses. These results provide the first evidence that, in adult brain, synaptic integrins activate local kinase cascades with potent effects on the operation of nearby neurotransmitter receptors implicated in synaptic plasticity.
|Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions.|
Matsuya, M, et al.
J. Biol. Chem., 273: 1003-14 (1998) 1998
Cell adhesion kinase beta (CAKbeta/PYK2) is the second protein-tyrosine kinase of the focal adhesion kinase subfamily. We identified a cDNA that encodes a CAKbeta-binding protein. This cDNA clone encodes the human homologue of Hic-5, the cDNA of which was cloned in 1994 as transforming growth factor beta1- and hydrogen peroxide-inducible mRNA. We found that Hic-5 exclusively localized at focal adhesions in a rat fibroblast line, WFB. This localization of Hic-5 was confirmed in WFB cells expressing Myc-tagged Hic-5. The amino acid sequence of Hic-5 is highly similar to that of paxillin in the four LD motifs as well as in the four contiguous LIM domains. The Hic-5 N-terminal domain directly associated in vitro with the extreme C-terminal region (residue 801 to the end) of CAKbeta. CAKbeta was coimmunoprecipitated with Hic-5 from the WFB cell lysate. The coimmunoprecipitation of CAKbeta with Hic-5 was markedly inhibited by the addition of the extreme C-terminal region of CAKbeta. Coimmunoprecipitation of Hic-5 with CAKbeta, which was shown in COS-7 cells doubly transfected with cDNA constructs of CAKbeta and Myc-tagged Hic-5, was lost when the CAKbeta amino acid residues 741-903 were deleted. Hic-5 was tyrosine-phosphorylated in Src-transformed 3Y1 cells and in cells treated with pervanadate. Hic-5 associated with CAKbeta was selectively tyrosine-phosphorylated in WFB cells exposed to hypertonic osmotic stress. These results indicate that Hic-5 is a paxillin-related component of focal adhesions and binds to CAKbeta, implying possible involvement of Hic-5 in the downstream signaling of CAKbeta.
|Differential signaling by the focal adhesion kinase and cell adhesion kinase beta|
Schaller, M.D. and Sasaki, T.
J. Biol. Chem., 272(40):25319-25325 (1997) 1997
|What is thiophilic chromatography?||Thiophilic chromatography is an alternative to protein A or protein G purification. Often used to purify IgM's or IgY's that do not have the affinity to protein A or G agarose. Actually, this purification method is able to be used for broad purification for all immunoglobulin types.|