05-488 | Anti-Pyk2/CAKβ Antibody, clone 74

05-488
250 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIP, WBMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-488
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Pyk2/CAKβ Antibody, clone 74
      Alternate Names
      • PTK2B
      • FAK2
      References
      Product Information
      FormatAscites
      Presentation250 μl of mouse ascites containing 0.05% sodium azide. Frozen at -20°C.
      Applications
      ApplicationAnti-Pyk2/CAKβ Antibody, clone 74 is an antibody against Pyk2/CAKβ for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 5-416 of rat Pyk2
      Clone74
      HostMouse
      SpecificityPyk2; does not recognize FAK
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
      Gene Symbol
      • PTK2B
      • PYK2
      • RAFTK
      • FADK2
      • PKB
      • FAK2
      • CAKB
      • PTK
      • CADTK
      Purification MethodThiophilic adsorption chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14289 # Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
      SIZE: 1009 amino acids; 115875 Da
      SUBUNIT: Interacts with Crk-associated substrate (Cas), PTPNS1 and SH2D3C (By similarity). Interacts with nephrocystin, DDEF2, OPHN1L, SKAP2 and TGFB1I1.
      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interaction with nephrocystin induces the membrane-association of the kinase.
      TISSUE SPECIFICITY: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
      PTM: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha- lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity (By similarity).
      SIMILARITY: SwissProt: Q14289 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight115kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from rat brain cytosol
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size250 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Pyk2/CAKB, clone 74 (mouse ascites) 2920716
      Anti-Pyk2/CAKb, clone 74 (mouse ascites) - 2025015 2025015
      Anti-Pyk2/CAKb, clone 74 (mouse ascites) - 2243476 2243476
      Anti-Pyk2/CAKb, clone 74 - 2043517 2043517
      Anti-Pyk2/CAKb, clone 74 - DAM1493398 DAM1493398
      Anti-Pyk2/CAKbeta;, clone 74 - 17028 17028
      Anti-Pyk2/CAKbeta;, clone 74 - 22940 22940
      Anti-Pyk2/CAKbeta;, clone 74 - 31834 31834
      Anti-Pyk2/CAKβ, clone 74 (mouse ascites) 2988096
      Anti-Pyk2/CAKβ, clone 74 -2772638 2772638

      References

      Reference overviewPub Med ID
      Integrin signaling cascades are operational in adult hippocampal synapses and modulate NMDA receptor physiology.
      Joie A Bernard-Trifilo, Enikö A Kramár, Reidun Torp, Ching-Yi Lin, Eduardo A Pineda, Gary Lynch, Christine M Gall
      Journal of neurochemistry  93  834-49  2005

      Show Abstract
      15857387 15857387
      Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions.
      Matsuya, M, et al.
      J. Biol. Chem., 273: 1003-14 (1998)  1998

      Show Abstract
      9422762 9422762
      Differential signaling by the focal adhesion kinase and cell adhesion kinase beta
      Schaller, M.D. and Sasaki, T.
      J. Biol. Chem., 272(40):25319-25325 (1997)  1997

      9312150 9312150

      FAQ

      QuestionAnswer
      What is thiophilic chromatography?Thiophilic chromatography is an alternative to protein A or protein G purification. Often used to purify IgM's or IgY's that do not have the affinity to protein A or G agarose. Actually, this purification method is able to be used for broad purification for all immunoglobulin types.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies