05-908 | Anti-RAD21 Antibody

05-908
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-908
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RAD21 Antibody
      OverviewAka Scc1, part of the cohesin complex, Rad21 is a nuclear protein that associates with mitotic chromatin and is essential for the association of kinetochores with microtubules. Rad21/Scc1 s is cleaved by caspases, promoting apoptosis.
      Background InformationThe protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
      RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
      PresentationPrtotein G purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4
      Applications
      ApplicationDetect RAD21 with Anti-RAD21 Antibody (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 240-631 of human Rad21
      HostMouse
      SpecificityRad21
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
      Gene Symbol
      • RAD21
      • NXP1
      • HR21
      • KIAA0078
      • FLJ40596
      • MCD1
      • hHR21
      • HRAD21
      • FLJ25655
      • SCC1
      • NXP-1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60216 # Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
      SIZE: 631 amino acids; 71690 Da
      SUBUNIT: Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Found in a complex with SMC1A, SMC3, CDCA5, PDS5A/APRIN and PDS5B/SCC-112.
      SUBCELLULAR LOCATION: Nucleus. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
      DOMAIN: SwissProt: O60216 The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3 (By similarity).
      PTM: Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites. & Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.
      SIMILARITY: Belongs to the rad21 family.
      Molecular Weight100-120kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot with HeLa nuclear extracts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RAD21 - 30391 30391
      Anti-Rad21, clone 53A303 2476729
      Anti-Rad21, clone 53A303 2483893
      Anti-Rad21, clone 53A303 (mouse monoclonal IgG) 2957954
      Anti-Rad21, clone 53A303 (mouse monoclonal IgG) 2880726
      Anti-Rad21, clone 53A303 (mouse monoclonal IgG) 2918902
      Anti-Rad21, clone 53A303 (mouse monoclonal IgG) - 2189942 2189942
      Anti-Rad21, clone 53A303 (mouse monoclonal IgG) - 2352367 2352367
      Anti-Rad21, clone 53A303 - 2031426 2031426
      Anti-Rad21, clone 53A303 - 2535820 2535820

      References

      Reference overviewApplicationPub Med ID
      Architectural proteins CTCF and cohesin have distinct roles in modulating the higher order structure and expression of the CFTR locus.
      Gosalia, N; Neems, D; Kerschner, JL; Kosak, ST; Harris, A
      Nucleic acids research  42  9612-22  2014

      Show Abstract
      25081205 25081205
      Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.
      Buheitel, J; Stemmann, O
      The EMBO journal  32  666-76  2013

      Show Abstract
      Western Blotting23361318 23361318
      Scc1 sumoylation by Mms21 promotes sister chromatid recombination through counteracting Wapl.
      Wu, N; Kong, X; Ji, Z; Zeng, W; Potts, PR; Yokomori, K; Yu, H
      Genes & development  26  1473-85  2012

      Show Abstract
      22751501 22751501
      Cohesin regulates MHC class II genes through interactions with MHC class II insulators.
      Majumder, P; Boss, JM
      Journal of immunology (Baltimore, Md. : 1950)  187  4236-44  2011

      Show Abstract
      21911605 21911605
      CTCF controls expression and chromatin architecture of the human major histocompatibility complex class II locus.
      Majumder, P; Boss, JM
      Molecular and cellular biology  30  4211-23  2010

      Show Abstract
      Immunoprecipitation20584980 20584980

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      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies