Key Spec Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H, M||WB||M||Purified||Monoclonal Antibody|
|Description||Anti-RAP1 Antibody, clone 4C8/1|
|Application||Anti-RAP1 Antibody, clone 4C8/1 is a high quality Mouse Monoclonal Antibody for the detection of RAP1 & has been validated in WB.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||stable 2 years at -20°C from date of shipment|
|Material Size||100 µg|
|Anti-RAP1, clone 4C8/1 - 30404||30404|
|Anti-Rap1, clone 4C8/1||2476730|
|Reference overview||Pub Med ID|
|Telomere stability genes are not mutated in osteosarcoma cell lines.|
Savage, Sharon A, et al.
Cancer Genet. Cytogenet., 160: 79-81 (2005) 2005
Osteosarcoma (OS), the most common primary bone tumor in adolescents and young adults, is characterized by a high degree of chromosomal abnormalities. Because telomeres are important for maintaining chromosomal integrity, it is plausible that germ-line or somatic mutations in the genes responsible for stabilizing the telomere complex could contribute to OS. We performed bi-directional sequence analysis in five OS cell lines and targeted all exons and proximal promoter regions in eight genes important in telomere stability: telomerase, the RNA component of telomerase (TERC), telomeric repeat binding factor 1, telomeric repeat binding factor 2, TERF1 interacting nuclear factor 2, human Rap1, protection of telomeres 1 and tankyrase. In this pilot study, we did not identify either somatic mutations or novel germ-line mutations in the five cell lines studied. However, we did confirm common genetic polymorphisms; an analysis of heterozygous sites suggests that loss of heterozygosity in OS is not present across these eight genes.