05-952 | Anti-RNA Polymerase II Antibody, CTD, clone 8WG16

05-952
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Yeast (S. cerevisiae)ICC, IP, WBMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-952
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RNA Polymerase II Antibody, CTD, clone 8WG16
      Alternate Names
      • DNA directed RNA polymerase II polypeptide A
      • DNA-directed RNA polymerase II largest subunit
      • RNA polymerase II 220
        kd subunit
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA polymerase II subunit B1
      • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
      • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
      Background InformationAs the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity
      References
      Product Information
      FormatAscites
      Control
      • HeLa nuclear extracts.

        Included Positive Antigen Control:
        Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
      PresentationMouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-RNA Polymerase II Antibody, CTD, clone 8WG16 (Mouse Monoclonal Antibody) validated in ChIP, ICC, IP, WB to detect RNA Polymerase II also known as DNA directed RNA polymerase II polypeptide A.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      Recommended by an independent laboratory.

      Immunocytochemistry:
      Recommended by an independent laboratory.
      Biological Information
      ImmunogenRNA Polymerase II from wheat germ extract. Clone 8WG16.
      Clone8WG16
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes RNA Polymerase II, Mr 220 kDa.
      Species Reactivity
      • Human
      • Yeast (S. cerevisiae)
      Species Reactivity NoteHuman and yeast. Broad species cross-reactivity is expected based on sequence conservation of immunogen.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
      Gene Symbol
      • POLR2A
      • POLRA
      • RpIILS
      • POLR2
      • RPB1
      • MGC75453
      • hsRPB1
      • RPOL2
      • RPBh1
      • RPO2
      • hRPB220
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24928 # DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
      Molecular Weight220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot.

      Western Blot Analysis:
      1:500-1:2000 dilution of this lot detected RNA Polymerase II in HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNA Polymerase II, CTD, clone 8WG16 - 2137080 2137080
      Anti-RNA Polymerase II, CTD, clone 8WG16 - 2366504 2366504
      Anti-RNA Polymerase II, CTD, clone 8WG16 - 2424635 2424635
      Anti-RNA Polymerase II, CTD, clone 8WG16 - DAM1797932 DAM1797932
      Anti-RNA Polymerase II, CTD, -2519219 2519219
      Anti-RNA Polymerase II, CTD, -2548160 2548160
      Anti-RNA Polymerase II, CTD, -2620625 2620625
      Anti-RNA Polymerase II, CTD, -2629272 2629272
      Anti-RNA Polymerase II, CTD, -2653120 2653120
      Anti-RNA Polymerase II, CTD, -2701525 2701525

      References

      Reference overviewApplicationSpeciesPub Med ID
      Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline.
      Sin, HS; Kartashov, AV; Hasegawa, K; Barski, A; Namekawa, SH
      BMC biology  13  53  2015

      Show Abstract
      26198001 26198001
      Mechanism of Action of 2-Aminobenzamide HDAC Inhibitors in Reversing Gene Silencing in Friedreich's Ataxia.
      Soragni, E; Chou, CJ; Rusche, JR; Gottesfeld, JM
      Frontiers in neurology  6  44  2015

      Show Abstract
      25798128 25798128
      The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis.
      Kuwahara, M; Suzuki, J; Tofukuji, S; Yamada, T; Kanoh, M; Matsumoto, A; Maruyama, S; Kometani, K; Kurosaki, T; Ohara, O; Nakayama, T; Yamashita, M
      Nature communications  5  3555  2014

      Show Abstract
      Mouse24694524 24694524
      Thyroid hormone signaling in vivo requires a balance between coactivators and corepressors.
      Vella, KR; Ramadoss, P; Costa-E-Sousa, RH; Astapova, I; Ye, FD; Holtz, KA; Harris, JC; Hollenberg, AN
      Molecular and cellular biology  34  1564-75  2014

      Show Abstract
      Western Blotting24550004 24550004
      The chromatin remodeling factor CHD5 is a transcriptional repressor of WEE1.
      Quan, J; Adelmant, G; Marto, JA; Look, AT; Yusufzai, T
      PloS one  9  e108066  2014

      Show Abstract
      25247294 25247294
      Network of mutually repressive metastasis regulators can promote cell heterogeneity and metastatic transitions.
      Lee, J; Lee, J; Farquhar, KS; Yun, J; Frankenberger, CA; Bevilacqua, E; Yeung, K; Kim, EJ; Balázsi, G; Rosner, MR
      Proceedings of the National Academy of Sciences of the United States of America  111  E364-73  2014

      Show Abstract
      24395801 24395801
      Deletion of a conserved cis-element in the Ifng locus highlights the role of acute histone acetylation in modulating inducible gene transcription.
      Balasubramani, A; Winstead, CJ; Turner, H; Janowski, KM; Harbour, SN; Shibata, Y; Crawford, GE; Hatton, RD; Weaver, CT
      PLoS genetics  10  e1003969  2014

      Show Abstract
      24415943 24415943
      Bi-functional cross-linking reagents efficiently capture protein-DNA complexes in Drosophila embryos.
      Aoki, T; Wolle, D; Preger-Ben Noon, E; Dai, Q; Lai, EC; Schedl, P
      Fly  8  43-51  2014

      Show Abstract
      24135698 24135698
      Optimal histone H3 to linker histone H1 chromatin ratio is vital for mesodermal competence in Xenopus.
      Lim, CY; Reversade, B; Knowles, BB; Solter, D
      Development (Cambridge, England)  140  853-60  2013

      Show Abstract
      Western Blotting23318639 23318639
      Epigenetic regulation of autophagy by the methyltransferase G9a.
      Artal-Martinez de Narvajas, A; Gomez, TS; Zhang, JS; Mann, AO; Taoda, Y; Gorman, JA; Herreros-Villanueva, M; Gress, TM; Ellenrieder, V; Bujanda, L; Kim, DH; Kozikowski, AP; Koenig, A; Billadeau, DD
      Molecular and cellular biology  33  3983-93  2013

      Show Abstract
      23918802 23918802

      Related Products & Applications

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies