05-623-AF488 | Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate

05-623-AF488
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-623-AF488
      DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate
      Alternate Names
      • DNA-directed RNA polymerase II subunit RPB1
      • RNA polymerase II subunit B1
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA-directed RNA polymerase II subunit RPB1
      • RNA polymerase II
      Background InformationDNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Applications
      ApplicationThis Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells.
      The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
      Epitopecarboxyl-terminal domain of RNA polymerase II
      CloneCTD4H8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis antibody recognizes Phospho- and non-phospho-RNA polymerase II.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Yeast, Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POLR2A
      • POLR2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~210-220 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies