05-623-Z | Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free

05-623-Z
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, YeastWB, ICC, ChIP-seqMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-623-Z
      DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free
      Alternate Names
      • DNA-directed RNA polymerase II subunit RPB1
      • RNA polymerase II subunit B1
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA-directed RNA polymerase II subunit RPB1
      Background InformationRNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. (This product is Ascites Free.)
      Applications
      ApplicationAnti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free is a highly specific mouse monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting, ICC & ChIP-seq.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ChIP-seq
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected RNA polymerase II in 10 µg of HeLa cell lysate.

      Immunocytochemistry Analysis: A 1:50 and 1:250 dilution from a representative lot detected RNA polymerase II in HeLa and A431 cells, respectively.
      Biological Information
      ImmunogenLinear peptide corresponding to human RNA polymerase II.
      CloneCTD4H8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Yeast
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POLR2A
      • POLR2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~220 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected RNA polymerase II in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNA polymerase II, clone -2707237 2707237
      Anti-RNA polymerase II, clone -2739008 2739008
      Anti-RNA polymerase II, clone CTD4H8, Ascites Free - Q2299332 Q2299332
      Anti-RNA polymerase II, clone CTD4H8, Ascites Free -2527083 2527083
      Anti-RNA polymerase II, clone CTD4H8, Ascites Free -2617689 2617689
      Anti-RNA polymerase II, clone CTD4H8, Ascites Free -2649262 2649262
      Anti-RNA polymerase II, clone CTD4H8, Ascites Free Monoclonal Antibody 2982397
      Anti-RNA polymerase II, clone CTD4H8, Ascites Free_2789065 2789065

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies