09-724 | Anti-RNF110/PCGF2 Antibody

09-724
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, BWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-724
      DescriptionAnti-RNF110/PCGF2 Antibody
      Alternate Names
      • DNA-binding protein Mel-18
      • RING finger protein 110
      • Zinc finger protein 144
      • polycomb group ring finger 2
      Background InformationPolycomb group ring finger 2, also known as PCGF2, is a transcriptional repressor. Polycomb group (PcG) of genes were first identified in Drosophila whereby these genes were required to sustain the stable repression of Hox cluster genes during development. Studies have shown that PCGF2 forms multimeric protein complexes associated with chromatin. In addition, PCGF2 may play a role in cell proliferation and/or neural cell development. PCGF2 is widely expressed in placenta, lung, and kidneys but expressed very little in skeletal muscle, liver, and pancreas.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human lung tissue lysate
      PresentationPurifeid rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RNF110/PCGF2 Antibody is a Rabbit Polyclonal Antibody for detection of RNF110/PCGF2 also known as DNA-binding protein Mel-18, RING finger protein 110 & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: 2 µg/mL from a previous lot detected RNF110/PCGF2 in Western Blot (SNAP ID) Analysis.
      Biological Information
      ImmunogenKLH conjugated linear peptide corresponding to human RNF110/PCGF2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes RNF110/PCGF2.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with bovine, horse, chimpanzee and macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses.
      Gene Symbol
      • MEL-18
      • MEL18
      • MGC10545
      • RNF110
      • ZNF144
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
      SUBUNIT STRUCTURE: Exists as both a monomer and homodimer By similarity. Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.
      PTM: Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers By similarity.
      SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.
      Molecular Weight~40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot in human lung tissue lysate.
      Western Blot Analysis: 1 µg/mL of this antibody detected RNF110/PCGF2 in 10 µg of human lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications