06-1355 | Anti-RNMT (mRNA methyltransferase) Antibody

06-1355
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1355
      DescriptionAnti-RNMT (mRNA methyltransferase) Antibody
      Alternate Names
      • mRNA cap guanine-N7 methyltransferase
      • RG7MT1
      • mRNA (guanine-N(7)-)-methyltransferase
      • mRNA cap methyltransferase
      • hCMT1
      • hMet
      • hcm1p
      Background InformationRNMT (mRNA methyltransferase) functions to methylate the mRNA guanosine cap during mRNA translation. Higher expression of RNMT correlates with increased cellular mRNA cap methyltransferase activity, activates epithelial cell transformation in mammals and increases cell transformation in fibroblasts. RNMT is also used to phosphorylate the RNA polymerase II CTD.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RNMT (mRNA methyltransferase) Antibody is a rabbit polyclonal antibody for detection of RNMT also known as mRNA cap guanine-N7 methyltransferase, RG7MT1, hCMT1, hMet, hcm1p & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 0.1 µg/mL from a representative lot detected RNMT on 10 µg of L6 and NIH/3T3 cell lysates.

      Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RNMT in HeLa, A431, and NIH/3T3 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human RNMT.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNMT
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

      SUBUNIT STRUCTURE: Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT.

      SUBCELLULAR LOCATION: Nucleus

      TISSUE SPECIFICITY: Widely expressed.

      SEQUENCE SIMILARITIES: Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.
      Molecular Weight~52 kDa observed. Uniprot describes 2 isoforms at 55 and 58 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected RNMT on 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNMT (mRNA methyltransferase) - 2205013 2205013
      Anti-RNMT (mRNA methyltransferase) - 2291759 2291759
      Anti-RNMT (mRNA methyltransferase) - NRG1915396 NRG1915396
      Anti-RNMT (mRNA methyltransferase) Polyclonal Antibody 2866725
      Anti-RNMT (mRNA methyltransferase) Polyclonal Antibody 2924632

      References

      Reference overviewPub Med ID
      Tubulin transport in neurons.
      K E Miller,H C Joshi
      The Journal of cell biology  133  1996

      Show Abstract Full Text Article
      8682870 8682870

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies