04-1121 | Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal

04-1121
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIH(P), WB, ICC, IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1121
      DescriptionAnti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal
      Alternate Names
      • Renal carcinoma antigen NY-REN-35
      • Rho-associated, coiled-coil containing protein kinase 1
      • p160 ROCK-1
      Background InformationRho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.
      References
      Product Information
      FormatUnpurified
      Control
      • HeLa cell lysate.
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-glycine (pH 7.4), 0.15 M NaCl containing 40% glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect ROCK-1 using this Anti-ROCK-1 Antibody, clone EPR638Y, Rabbit validated for use in IH(P), WB, IC & IP.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: 1:50-100 dilution from a previous lot detected ROCK-1 in human lymphoma tissue.
      Immunocytochemistry: 1:50-100 dilution from a previous lot was used in IC.
      Immunoprecipitation: 1:60 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues near the C-terminus of human ROCK-1.
      EpitopeC-terminus
      CloneEPR638Y
      HostRabbit
      SpecificityThis antibody recognizes ROCK-1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC 2.7.11.1
      • MGC131603
      • MGC43611
      • OTTHUMP00000162514
      • P160ROCK
      • PRO0435
      • p160-ROCK
      • p160ROCK
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis By similarity.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION: Activated by RHOA binding.
      SUBUNIT STRUCTURE: Binds RHOA (activated by GTP). Interacts with ADD1, GEM, RHOB, RHOC, MYLC2B and VIM By similarity. Binds RHOE, PPP1R12A, LIMK1 and LIMK2. Interacts with TSG101.
      SUBCELLULAR LOCATIION: Cytoplasm. Centrosome › centriole By similarity. Golgi apparatus membrane; Peripheral membrane protein. Note: Associated with the mother centriole and an intercentriolar linker By similarity. A small proportion is associated with Golgi membranes.
      TISSUE SPECIFICITY: Detected in blood platelets.
      DOMAIN: The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
      PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
      MISCELLANEOUS: Inhibited by Y-27632.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
      Contains 1 REM (Hr1) repeat.
      Molecular Weight~ 160 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on HeLa cell lysates.
      Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect ROCK-1 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - 2366005 2366005
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - 2424669 2424669
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal 2462780
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - 2203074 2203074
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - 2271207 2271207
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - 2298419 2298419
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - 2329162 2329162
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - 2356332 2356332
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - NG1625686 NG1625686
      Anti-ROCK-1, clone EPR638Y, Rabbit Monoclonal - NG1749698 NG1749698

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies