04-841 | Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

04-841
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, BIP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-841
      Replaces05-841
      DescriptionAnti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
      Alternate Names
      • RhoA-binding kinase 2
      • Rho-associated, coiled-coil containing protein kinase 2
      • Rho-associated, coiled-coil-containing protein kinase II
      • p150 ROK-alpha
      • p164 ROCK-2
      • ROCK-II
      • ROKalpha
      • Rock2
      Background InformationRho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • RIPA lysates from PC-12 cells
      PresentationCultured supernatant containing 0.05% sodium azide.
      Applications
      ApplicationDetect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
      Biological Information
      Immunogen6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.
      CloneA9W4
      HostRabbit
      SpecificityRecognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      Species Reactivity NoteHuman and rat. Predicted to cross-react with mouse and bovine based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA Rho kinase that is involved in inducing membrane blebbing and chromatin condensation [RGD].
      Gene Symbol
      • EC 2.7.11.1
      • MGC131603
      • MGC43611
      • OTTHUMP00000162514
      • P160ROCK
      • PRO0435
      UniProt Number
      UniProt SummaryFUNCTION: Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis By similarity.

      Catalytic activity ATP + a protein = ADP + a phosphoprotein.

      Enzyme regulation Activated by RHOA binding.

      SUBUNIT: Binds RHOA that has been activated by GTP binding. Binds ADD1, GEM, RHOB, RHOC, myosin light chain and VIM By similarity. Binds RHOE, PPP1R12A (MYPT1/MBS), LIMK1 and LIMK2.

      SUBCELLULAR LOCATION: Cytoplasm. Centrosome › centrioleBy similarity. Golgi apparatus membrane; Peripheral membrane protein. Note= Associated with the mother centriole and an intercentriolar linker By similarity. A small proportion is associated with Golgi membranes.

      TISSUE SPECIFICITY: Detected in blood platelets. Ref.1

      DOMAIN: The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.

      PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.1 Ref.10 Ref.11 Ref.12

      Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

      Miscellaneous Inhibited by Y-27632.

      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

      Contains 1 AGC-kinase C-terminal domain.

      Contains 1 PH domain.

      Contains 1 phorbol-ester/DAG-type zinc finger.

      Contains 1 protein kinase domain.

      Contains 1 REM (Hr1) repeat.

      Molecular Weight150 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western BLot on RIPA lysates from PC-12 cells.

      Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ROK#945;/ROCK-II, clone A9W4 - 2159666 2159666
      Anti-ROK/ROCK-II, clone A9W4 - 2274268 2274268
      Anti-ROK/ROCK-II, clone A9W4 - 2330576 2330576
      Anti-ROKα/ROCK-II, clone A9W4 - 2427494 2427494
      Anti-ROKα/ROCK-II, clone A9W4 -2517790 2517790
      Anti-ROKα/ROCK-II, clone A9W4 -2604785 2604785
      Anti-ROKα/ROCK-II, clone A9W4 -2806108 2806108

      References

      Reference overviewPub Med ID
      The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization.
      Vincent, S and Settleman, J
      Mol. Cell. Biol., 17: 2247-56 (1997)  1997

      Show Abstract
      9121475 9121475
      The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton.
      Leung, T, et al.
      Mol. Cell. Biol., 16: 5313-27 (1996)  1996

      Show Abstract
      8816443 8816443