05-1574 | Anti-Rab25 Antibody, clone 12C3

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbWB, IHCMPurifiedMonoclonal Antibody
      Catalogue Number05-1574
      DescriptionAnti-Rab25 Antibody, clone 12C3
      Alternate Names
      • RAB25, member RAS oncogene family
      • ras-related protein Rab-25
      Background InformationRas-related protein Rab-25 (Rab25) is a GTP-binding protein belonging to the Rab family of proteins. Rab25 has observed expression in kidney, lung and gastrointestinal mucosa, appearing to be exclusively expressed within epithelial cells. This specific expression has led to the implication that Rab25 has a role as a regulator for vesicular transport associated with epithelia. Rab25 is potentially a selective regulator for transcytotic pathways and apical recycling. Several recent studies have indicated an observable loss of Rab25 in breast cancer tissue and cell lines, and have suggested a potential role for Rab25 as a regulator for Ras induced tumorigenesis.
      Product Information
      • MCF-7 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.
      ApplicationAnti-Rab25 Antibody, clone 12C3 is a Mouse Monoclonal Antibody for the detection of Rab25 & has been validated in WB & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Goldenring, J. R. et al. (1996). Am. J. Physiol. 270(33):G515-G525.)

      Imunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Casanova, J.E. et al. (1999). Molecular Biology of the Cell. 10:47-61.)
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to the C-terminus of rabbit Rab25.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Rab25 at the C-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse, rat, and rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAB25
      • CATX-8
      • RAB11C
      • CATX8
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: May selectively regulate the apical recycling and/or transcytotic pathways.

      SUBUNIT STRUCTURE: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4 (By similarity).

      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

      TISSUE SPECIFICTY: Expressed in the gastrointestinal mucosa, (highest expression seen in the ileum and colon), kidney, and lung. A very minor and variable level of expression is seen in the splenic tissue.

      SEQUENCE SIMLARITIES: Belongs to the small GTPase superfamily. Rab family.
      Molecular Weight~ 25 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Rab25 on 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information