05-389 | Anti-Rac1 Antibody, clone 23A8

05-389
250 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-389
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Rac1 Antibody, clone 23A8
      Background InformationRac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.
      References
      Product Information
      FormatPurified
      Control
      • Immunoblot/Immunoprecipitation: Rat brain microsomal protein preparation
        Immunohistochemistry: Rat brain sections
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-Rac1 Antibody, clone 23A8 has been published in more than 40 citations and validated for use in Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Rac1 protein.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein containing the full length human Rac
      Clone23A8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
      Gene Symbol
      • RAC1
      • MGC111543
      • ORFNames=MIG5
      • MIG5
      • TC-25
      • p21-Rac1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
      SIZE: 192 amino acids; 21450 Da
      SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
      DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.
      SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
      Molecular Weight21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on rat brain microsomal protein preparation
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Rac1, clone 23A8 2476975
      Anti-Rac1, clone 23A8 - 2116619 2116619
      Anti-Rac1, clone 23A8 - 2382127 2382127
      Anti-Rac1, clone 23A8 - 2397127 2397127
      Anti-Rac1, clone 23A8 - 2452510 2452510
      Anti-Rac1, clone 23A8 - 15254 15254
      Anti-Rac1, clone 23A8 - 16999 16999
      Anti-Rac1, clone 23A8 - 18070 18070
      Anti-Rac1, clone 23A8 - 18423 18423
      Anti-Rac1, clone 23A8 - 18826 18826

      References

      Reference overviewApplicationSpeciesPub Med ID
      Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms.
      Hinterseher, I; Schworer, CM; Lillvis, JH; Stahl, E; Erdman, R; Gatalica, Z; Tromp, G; Kuivaniemi, H
      International journal of molecular sciences  16  11196-212  2015

      Show Abstract
      25993291 25993291
      The proto-oncogene c-Src and its downstream signaling pathways are inhibited by the metastasis suppressor, NDRG1.
      Liu, W; Yue, F; Zheng, M; Merlot, A; Bae, DH; Huang, M; Lane, D; Jansson, P; Lui, GY; Richardson, V; Sahni, S; Kalinowski, D; Kovacevic, Z; Richardson, DR
      Oncotarget  6  8851-74  2015

      Show Abstract
      25860930 25860930
      Lichen Secondary Metabolite, Physciosporin, Inhibits Lung Cancer Cell Motility.
      Yang, Y; Park, SY; Nguyen, TT; Yu, YH; Nguyen, TV; Sun, EG; Udeni, J; Jeong, MH; Pereira, I; Moon, C; Ha, HH; Kim, KK; Hur, JS; Kim, H
      PloS one  10  e0137889  2015

      Show Abstract
      26371759 26371759
      Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
      Poitelon, Y; Bogni, S; Matafora, V; Della-Flora Nunes, G; Hurley, E; Ghidinelli, M; Katzenellenbogen, BS; Taveggia, C; Silvestri, N; Bachi, A; Sannino, A; Wrabetz, L; Feltri, ML
      Nature communications  6  8303  2015

      Show Abstract
      Immunohistochemistry26383514 26383514
      ROCK1 deficiency enhances protective effects of antioxidants against apoptosis and cell detachment.
      Surma, M; Handy, C; Chang, J; Kapur, R; Wei, L; Shi, J
      PloS one  9  e90758  2014

      Show Abstract
      Western Blotting24595357 24595357
      Impaired cell death and mammary gland involution in the absence of Dock1 and Rac1 signaling.
      Bagci, H; Laurin, M; Huber, J; Muller, WJ; Côté, JF
      Cell death & disease  5  e1375  2014

      Show Abstract
      25118935 25118935
      Downregulation of microRNA-100 enhances the ICMT-Rac1 signaling and promotes metastasis of hepatocellular carcinoma cells.
      Zhou, HC; Fang, JH; Luo, X; Zhang, L; Yang, J; Zhang, C; Zhuang, SM
      Oncotarget  5  12177-88  2014

      Show Abstract
      Western BlottingMouse25361001 25361001
      Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
      Moon, S; Han, D; Kim, Y; Jin, J; Ho, WK; Kim, Y
      Scientific reports  4  4376  2014

      Show Abstract
      Western Blotting24625528 24625528
      Epithelial splicing regulatory proteins 1 (ESRP1) and 2 (ESRP2) suppress cancer cell motility via different mechanisms.
      Ishii, H; Saitoh, M; Sakamoto, K; Kondo, T; Katoh, R; Tanaka, S; Motizuki, M; Masuyama, K; Miyazawa, K
      The Journal of biological chemistry  289  27386-99  2014

      Show Abstract
      25143390 25143390
      VAV3 mediates resistance to breast cancer endocrine therapy.
      Aguilar, H; Urruticoechea, A; Halonen, P; Kiyotani, K; Mushiroda, T; Barril, X; Serra-Musach, J; Islam, A; Caizzi, L; Di Croce, L; Nevedomskaya, E; Zwart, W; Bostner, J; Karlsson, E; Pérez Tenorio, G; Fornander, T; Sgroi, DC; Garcia-Mata, R; Jansen, MP; García, N; Bonifaci, N; Climent, F; Soler, MT; Rodríguez-Vida, A; Gil, M; Brunet, J; Martrat, G; Gómez-Baldó, L; Extremera, AI; Figueras, A; Balart, J; Clarke, R; Burnstein, KL; Carlson, KE; Katzenellenbogen, JA; Vizoso, M; Esteller, M; Villanueva, A; Rodríguez-Peña, AB; Bustelo, XR; Nakamura, Y; Zembutsu, H; Stål, O; Beijersbergen, RL; Pujana, MA
      Breast cancer research : BCR  16  R53  2014

      Show Abstract
      24886537 24886537

      Brochure

      Title
      Western Blotting Tools

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-389-AF488 Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 488 Conjugate Show Pricing & Availability
      05-389-AF647 Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate Show Pricing & Availability
      07-1464 Anti-Rac1 Antibody Show Pricing & Availability
      07-896 Anti-phospho-Rac1/cdc42 (Ser71) Antibody Show Pricing & Availability
      07-896-I Anti-phospho-Rac1/cdc42 (Ser71) Antibody Show Pricing & Availability
      09-271 Anti-Rac1b Antibody Show Pricing & Availability
      16-319 Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate Show Pricing & Availability
      16-637 Anti-Rac1 Antibody, clone 23A8, Agarose conjugate Show Pricing & Availability
      FCMAB330F Milli-Mark™ Anti-Rac1-FITC Antibody, clone 23A8 Show Pricing & Availability

      Accessories

      Catalogue Number Description  
      09-257 Anti-phospho-PAK1 (Ser204) Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies