05-530 | Anti-Rad51 Antibody, clone 3C10

05-530
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-530
      ReplacesMABE515
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Rad51 Antibody, clone 3C10
      Background InformationRad51 is similar to RecA, both biochemically and structurally. It promotes homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). Recent studies suggested that Rad51 is involved not only in homologous recombination but also in cell proliferation regulation.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell nuclear extract
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect Rad51 with Anti-Rad51 Antibody, clone 3C10 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenFull length human Rad51
      Cloneclone 3C10
      HostMouse
      SpecificityRad51; slight cross-reactivity with DMC1
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.
      Gene Symbol
      • RAD51
      • RAD51L3
      • hRAD51
      • HRAD51
      • RECA
      • RAD51D
      • RAD51A
      • HsT16930
      • BRCC5
      • HsRad51
      • HsRAD51
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q06609 # May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.
      SIZE: 339 amino acids; 36966 Da
      SUBUNIT: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1.
      SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage.
      TISSUE SPECIFICITY: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
      PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.DISEASE:SwissProt: Q06609 # Defects in RAD51 are associated with breast cancer (BC) [MIM:114480].
      SIMILARITY: SwissProt: Q06609 ## Belongs to the recA family. RAD51 subfamily. & Contains 1 HhH domain.
      Molecular Weight37kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Rad51, clone 3C10 (mouse monoclonal IgG1) - 1963625 1963625
      Anti-Rad51, clone 3C10 (mouse monoclonal IgG1) - 2322510 2322510
      Anti-Rad51, clone 3C10 (mouse monoclonal IgG1) - DAM1824761 DAM1824761
      Anti-Rad51, clone 3C10 (mouse monoclonal IgG1) - JBC1941194 JBC1941194
      Anti-Rad51, clone 3C10 (mouse monoclonal IgG1) -2491220 2491220
      Anti-Rad51, clone 3C10 - 19209 19209
      Anti-Rad51, clone 3C10 - 23426 23426
      Anti-Rad51, clone 3C10 - DAM1604954 DAM1604954
      Anti-Rad51, clone 3C10 - DAM1754390 DAM1754390
      Anti-Rad51, clone 3C10 - DAM1770279 DAM1770279

      References

      Reference overviewApplicationPub Med ID
      Elevated estrogen receptor-α in VHL-deficient condition induces microtubule organizing center amplification via disruption of BRCA1/Rad51 interaction.
      Jung, YS; Chun, HY; Yoon, MH; Park, BJ
      Neoplasia (New York, N.Y.)  16  1070-81  2014

      Show Abstract
      25499220 25499220
      Distinct functions of human RECQ helicases WRN and BLM in replication fork recovery and progression after hydroxyurea-induced stalling.
      Sidorova, JM; Kehrli, K; Mao, F; Monnat, R
      DNA repair  12  128-39  2013

      Show Abstract
      Western Blotting23253856 23253856
      Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.
      Cousin, W; Ho, ML; Desai, R; Tham, A; Chen, RY; Kung, S; Elabd, C; Conboy, IM
      PloS one  8  e63528  2013

      Show Abstract
      23704914 23704914
      Multiple possible sites of BRCA2 interacting with DNA repair protein RAD51.
      Katagiri, T, et al.
      Genes Chromosomes Cancer, 21: 217-22 (1998)  1998

      Show Abstract
      9523196 9523196
      Distribution of the Rad51 recombinase in human and mouse spermatocytes.
      Barlow, A L, et al.
      EMBO J., 16: 5207-15 (1997)  1997

      Show Abstract
      9311981 9311981
      Association of BRCA1 with Rad51 in mitotic and meiotic cells.
      Scully, R, et al.
      Cell, 88: 265-75 (1997)  1997

      Show Abstract
      9008167 9008167

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies