05-1470 | Anti-Raptor Antibody, clone 1H6.2

05-1470
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1470
      DescriptionAnti-Raptor Antibody, clone 1H6.2
      Alternate Names
      • P150 target of rapamycin (TOR)-scaffold protein
      • p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
      • regulatory associated protein of mTOR
      Background InformationRaptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.
      References
      Product Information
      FormatPurified
      Control
      • HEK 293 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Raptor Antibody, clone 1H6.2 detects level of Raptor & has been published & validated for use in WB, IP & IC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Raptor in NIH/3T3 cells.

      Immunoprecipitation Analysis: 20 µg from a previous lot immunoprecipitated Raptor in HEK293 cell lysate.
      Biological Information
      ImmunogenRecombinant protein corresponding to human Raptor.
      EpitopeUnknown
      Clone1H6.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Raptor.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • raptor
      • Raptor
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.

      SUBUNIT STRUCTURE: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains FRAP1, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Forms a complex with FRAP1 under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation.

      TISSUE SPECIFICTY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.

      SEQUENCE SIMILARITIES: Belongs to the WD repeat RAPTOR family.

      Contains 7 WD repeats.
      Molecular Weight~ 150 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: 1 µg/ml of this antibody detected Raptor on 10 µg of HEK 293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Raptor, clone 1H6.2 - 2397132 2397132
      Anti-Raptor, clone 1H6.2 - NG1833943 NG1833943
      Anti-Raptor, clone 1H6.2 - 1991286 1991286
      Anti-Raptor, clone 1H6.2 - 2061456 2061456
      Anti-Raptor, clone 1H6.2 - 2167189 2167189
      Anti-Raptor, clone 1H6.2 - 2291710 2291710
      Anti-Raptor, clone 1H6.2 - JBC1793843 JBC1793843
      Anti-Raptor, clone 1H6.2 - NG1741718 NG1741718
      Anti-Raptor, clone 1H6.2 - NG1853796 NG1853796
      Anti-Raptor, clone 1H6.2 - NG1894405 NG1894405

      References

      Reference overviewApplicationPub Med ID
      Loss of mTOR signaling affects cone function, cone structure and expression of cone specific proteins without affecting cone survival.
      Ma, S; Venkatesh, A; Langellotto, F; Le, YZ; Hall, MN; Rüegg, MA; Punzo, C
      Experimental eye research  135  1-13  2015

      Show Abstract
      25887293 25887293
      Host mTORC1 signaling regulates andes virus replication.
      McNulty, S; Flint, M; Nichol, ST; Spiropoulou, CF
      Journal of virology  87  912-22  2013

      Show Abstract
      Western Blotting23135723 23135723
      Pim2 is required for maintaining multiple myeloma cell growth through modulating TSC2 phosphorylation.
      Lu, J; Zavorotinskaya, T; Dai, Y; Niu, XH; Castillo, J; Sim, J; Yu, J; Wang, Y; Langowski, JL; Holash, J; Shannon, K; Garcia, PD
      Blood  122  1610-20  2013

      Show Abstract
      Immunoprecipitation23818547 23818547

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies