06-1105 | Anti-RelB Antibody

06-1105
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rhesus MacaqueWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1105
      Replaces04-1077
      DescriptionAnti-RelB Antibody
      Alternate Names
      • reticuloendotheliosis viral oncogene homolog B
      • v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
      • v-rel reticuloendotheliosis viral oncogene homolog B
      • v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in -cells 3
      Background InformationNFĸB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100). NFĸB/Rel proteins exist as homo- or heterodimer complexes. In a majority of unstimulated cells, the NFĸB transcription factors are bound to the inhibitory IĸB proteins and retained in the cytoplasm in their inactive form. RelB is a component of the NFĸB RelB-p50 complex and the RelB-p52 complex. RelB's interaction in complex seems to be transient and prevents degradation, allowing for heterodimer formation with p50 or p52.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Raji cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-RelB Antibody (rabbit polyclonal antibody) validated in WB,IHC(P) to detect RelB also known as reticuloendotheliosis viral oncogene homolog B, v-rel avian reticuloendotheliosis viral oncogene homolog B.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:400 dilution from a previous lot detected RelB in ductal carcinoma in situ tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human RelB at the RHD domain.
      EpitopeRHD domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes RelB at the RHD domain.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat.
      Predicted to react with rhesus macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • I-REL
      • I-Rel
      • IREL
      • RelB
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.

      SUBUNIT STRUCTURE: Component of the NF-kappa-B RelB-p50 complex. Component of the NF-kappa-B RelB-p52 complex. Self-associates; the interaction seems to be transient and may prevent degradation allowing for heterodimer formation with p50 or p52. Interacts with NFKB1/p50, NFKB2/p52 and NFKB2/p100. Interacts with NFKBID By similarity.

      SUBCELLULAR LOCATION: Nucleus.

      INDUCTION: By mitogens.

      DOMAIN: Both N- and C-terminal domains are required for transcriptional activation.

      PTM: Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation By similarity.

      SEQUENCE SIMILARITIES: Contains 1 RHD (Rel-like) domain.

      CAUTION: Was originally (Ref.1) thought to inhibit the transcriptional activity of nuclear factor NF-kappa-B.
      Molecular Weight~58 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Raji cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected RelB in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      04-1077 Anti-RelB (NT) Antibody, clone EP613Y, rabbit monoclonal Show Pricing & Availability
      07-1332 Anti-RelB (NFĸB family member) Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies