05-378 | Anti-RhoGAP p190 Antibody, clone D2D6

05-378
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, MkIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-378
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RhoGAP p190 Antibody, clone D2D6
      Alternate Names
      • CDC42 GTPase-activating protein
      • GTPase-activating protein rhoOGAP
      • Rho GTPase activating protein 1
      • Rho-related small GTPase protein activator
      • Rho-type GTPase-activating protein 1
      Background InformationRhoGAP p190 (Rho GTPase Activating Protein 5, ARHGAP5), like other RhoGAPs, stimulates the intrinsic GTPases activity of Rho, Rac, and Cdc42, but shows definite preference for Rho. RhoGAP p190 contains an N-terminal GTPase domain and a C-terminal RhoGAP domain. The activity of RhoGAP p190 is known to be regulated by phosphorylation, by kinases such as Src that is stimulated by growth factor signaling or cell attachment and phosphorylate RhoGAP p190 in its central region. This ultimately leads to reduced RHO-GTP and decreased stress fibers.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels.
      PresentationPurified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationDetect RhoGAP p190 using this Anti-RhoGAP p190 Antibody, clone D2D6 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated RhoGAP from 500 μg of human A431 carcinoma cell RIPA lysate.
      Biological Information
      ImmunogenGST-fusion protein to amino acids 180-610 of rat p190 RhoGAP.
      CloneD2D6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes RhoGAP, MW ~190kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARHGAP1
      • RHOGAP
      • p50-RhoGAP
      • CDC42GA1
      • CDC42GAP
      • Cdc42GAP
      • p50rhoGAP
      • RHOGAP1
      • RhoGAP
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q07960 # GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
      SIZE: 439 amino acids; 50436 Da
      SUBUNIT: Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with BNIPL.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Ubiquitous.
      SIMILARITY: SwissProt: Q07960 ## Contains 1 CRAL-TRIO domain. & Contains 1 Rho-GAP domain.
      Molecular Weight~190 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated in immunoblot on RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts or rabbit brain membrane extract.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected RhoGAP in RIPA lysates from human A431 carcinoma cells. Previous lots of the antibody detected RhoGAP in mouse 3T3 fibroblasts and a rabbit brain membrane extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RhoGAP p190, clone D2D6 2472927
      Anti-RhoGAP p190, clone D2D6 - 14724 14724
      Anti-RhoGAP p190, clone D2D6 - 17576 17576
      Anti-RhoGAP p190, clone D2D6 - 18411 18411
      Anti-RhoGAP p190, clone D2D6 - 21047 21047
      Anti-RhoGAP p190, clone D2D6 - 21917 21917
      Anti-RhoGAP p190, clone D2D6 - 23693 23693
      Anti-RhoGAP p190, clone D2D6 - 29504 29504
      Anti-RhoGAP p190, clone D2D6 -2511142 2511142

      References

      Reference overviewPub Med ID
      Control of neurite outgrowth by RhoA inactivation.
      Chan-Young Jeon,Mi-Young Moon,Jong-Hyun Kim,Hee-Jun Kim,Jae-Gyu Kim,Yi Li,Jae-Kwang Jin,Pyeung-Hyeun Kim,Hyoung-Chun Kim,Kathryn E Meier,Yong-Sun Kim,Jae-Bong Park
      Journal of neurochemistry  120  2012

      Show Abstract
      22035369 22035369
      Control of neural cell composition in poly(ethylene glycol) hydrogel culture with soluble factors.
      Mooney, R; Haeger, S; Lawal, R; Mason, M; Shrestha, N; Laperle, A; Bjugstad, K; Mahoney, M
      Tissue engineering. Part A  17  2805-15  2011

      Show Abstract
      21823990 21823990
      Cdk5-dependent regulation of Rho activity, cytoskeletal contraction, and epithelial cell migration via suppression of Src and p190RhoGAP.
      Tripathi, BK; Zelenka, PS
      Molecular and cellular biology  29  6488-99  2009

      Show Abstract Full Text Article
      19822667 19822667
      Ih without Kir in adult rat retinal ganglion cells.
      Sherwin C Lee,Andrew T Ishida
      Journal of neurophysiology  97  2007

      Show Abstract Full Text Article
      17488978 17488978
      p190 RhoGAP, the major RasGAP-associated protein, binds GTP directly.
      Foster, R, et al.
      Mol. Cell. Biol., 14: 7173-81 (1994)  1994

      Show Abstract
      7935432 7935432
      Association between GTPase activators for Rho and Ras families.
      Settleman, J, et al.
      Nature, 359: 153-4 (1992)  1992

      Show Abstract
      1522900 1522900
      Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus.
      Settleman, J, et al.
      Cell, 69: 539-49 (1992)  1992

      Show Abstract
      1581965 1581965
      Phosphorylation of GAP and GAP-associated proteins by transforming and mitogenic tyrosine kinases.
      Ellis, C, et al.
      Nature, 343: 377-81 (1990)  1990

      Show Abstract
      1689011 1689011

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies