05-1471 | Anti-Rictor Antibody, clone 9F1.2

05-1471
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1471
      DescriptionAnti-Rictor Antibody, clone 9F1.2
      Alternate Names
      • Rictor
      • AVO3
      • rapamycin-insensitive companion of mTOR
      • mAVO3
      Background InformationRictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysate.

        Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.
      PresentationPurified mouse monoclonal IgG2aκ ascites in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation:
      Representative lot data.
      1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.
      Biological Information
      ImmunogenRecombinant protein corresponding to human Rictor.
      EpitopeUnknown
      Clone9F1.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Rictor.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RICTOR
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummarySubunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. Ref.1 Ref.7 Ref.8
      Molecular Weight190 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting on 10 mg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Rictor, clone 9F1.2 - 2137033 2137033
      Anti-Rictor, clone 9F1.2 - 2455718 2455718
      Anti-Rictor, clone 9F1.2 - 05-1471 05-1471
      Anti-Rictor, clone 9F1.2 - 1964534 1964534
      Anti-Rictor, clone 9F1.2 - 2003026 2003026
      Anti-Rictor, clone 9F1.2 - 2108574 2108574
      Anti-Rictor, clone 9F1.2 - 2199691 2199691
      Anti-Rictor, clone 9F1.2 - 2266747 2266747
      Anti-Rictor, clone 9F1.2 - JH1769952 JH1769952
      Anti-Rictor, clone 9F1.2 - NG1862442 NG1862442

      References

      Reference overviewPub Med ID
      Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways.
      Wang, S; Amato, KR; Song, W; Youngblood, V; Lee, K; Boothby, M; Brantley-Sieders, DM; Chen, J
      Molecular and cellular biology  35  1299-313  2015

      Show Abstract
      25582201 25582201

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies