04-805 | Anti-SET7/9 Antibody, clone 5F2.3

04-805
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-805
      ReplacesMABE529
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SET7/9 Antibody, clone 5F2.3
      Alternate Names
      • SetD7
      • Histone-lysine N-methyltransferase SETD7
      • EC 2.1.1.43
      • SET domain-containing protein 7
      • SET7/9
      • Histone H3-K4 methyltransferase SETD7
      • Set7
      • Set9
      Background InformationThe methylation of histones plays a pivotal role in the regulation of chromatin structure and gene expression. Histone methylation can occur on Arg or Lys residues, with an exquisite site selectivity for Lys methylation at specific positions in the N-termini of histones H3 and H4. SET7/9, a histone methyltransferase (HMTase), which transfers methyl groups to Lys4 of Histone H3, forms a complex with S-adenosyl-L-methionine. This complex contains an active site consisting of a binding pocket where an AdoMet molecule, in an unusual conformation, binds a narrow substrate-specific channel that only unmethylated lysine residues can access and a catalytic tyrosine residue.
      References
      Product Information
      FormatPurified
      Presentation100 μg of protein G purified mouse IgG1κ in Tris-Glycine with 0.05% NaN3.
      Applications
      ApplicationUse Anti-SET7/9 Antibody, clone 5F2.3 (Mouse Monoclonal Antibody) validated in WB to detect SET7/9 also known as Histone-lysine N-methyltransferase SETD7, Set7, Set9.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenN-terminal His-tagged human SET9 recombinant protein
      EpitopeN-terminal region
      Clone5F2.3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes human SET7/9
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteNot yet tested in other species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      UniProt Number
      UniProt SummaryFUNCTION: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.

      SUBUNIT: Interacts with IPF1/PDX-1.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Widely expressed. Expressed in pancreatic islets.

      DOMAIN: The SET domain is necessary but not sufficient for histone methyltransferase activity.

      MISCELLANEOUS: Monomethyltransferase activity is achieved by disrupting the formation at near-attack conformations for the dimethylation reaction.
      Molecular Weight49 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from NIH/3T3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-SET7/9, clone 5F2.3 - 2344999 2344999
      Anti-SET7/9, clone 5F2.3 - 2015583 2015583
      Anti-SET7/9, clone 5F2.3 - 2216900 2216900
      Anti-SET7/9, clone 5F2.3 - 2495643 2495643
      Anti-SET7/9, clone 5F2.3 - NG1533186 NG1533186
      Anti-SET7/9, clone 5F2.3 - NG1734858 NG1734858
      Anti-SET7/9, clone 5F2.3 - NG1822435 NG1822435
      Anti-SET7/9, clone 5F2.3 - NG1869245 NG1869245
      Anti-SET7/9, clone 5F2.3 -2752544 2752544

      References

      Reference overviewApplicationPub Med ID
      The HPV E6 oncoprotein targets histone methyltransferases for modulating specific gene transcription.
      Hsu, CH; Peng, KL; Jhang, HC; Lin, CH; Wu, SY; Chiang, CM; Lee, SC; Yu, WC; Juan, LJ
      Oncogene  31  2335-49  2012

      Show Abstract
      Western Blotting21963854 21963854

      Related Products & Applications

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      Accessories

      Catalogue Number Description  
      14-469 SET9 Protein, Active, 100 µg Show Pricing & Availability
      14-539 PR-SET7 Protein, active - GST-tagged, 100 µg Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies