07-315 | Anti-SGK1 Antibody

07-315
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-315
      Replaces04-1027
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SGK1 Antibody
      References
      Product Information
      FormatPurified
      Control
      • Rat 2 cell lysate
      PresentationImmunoaffinity Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 1.75mg/ml BSA before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-SGK1 Antibody is validated for use in IP, WB for the detection of SGK1.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 399-412 (C-GKSPDSVLVTASVK) of human SGK1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes SGK1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.
      Gene Symbol
      • SGK
      • SGK1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O00141 # Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1.
      SIZE: 431 amino acids; 48942 Da
      SUBUNIT: Interacts with NEDD4 and NEDD4L.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Nuclear, upon phosphorylation.
      TISSUE SPECIFICITY: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung.
      PTM: Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3- kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. & Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells.
      SIMILARITY: SwissProt: O00141 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
      Molecular Weight~50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 and Rat 2 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SGK1 (rabbit polyclonal IgG) - 2383519 2383519
      Anti-SGK1 (rabbit polyclonal IgG) - 1993923 1993923
      Anti-SGK1 (rabbit polyclonal IgG) - 2202549 2202549
      Anti-SGK1 (rabbit polyclonal IgG) -2491165 2491165
      Anti-SGK1 - 22250 22250
      Anti-SGK1 - 26066 26066
      Anti-SGK1 - DAM1412900 DAM1412900
      Anti-SGK1 - DAM1413360 DAM1413360
      Anti-SGK1 - DAM1687545 DAM1687545
      Anti-SGK1 - DAM1776453 DAM1776453

      References

      Reference overviewApplicationPub Med ID
      Hippocampal CA1 transcriptional profile of sleep deprivation: relation to aging and stress.
      Porter, NM; Bohannon, JH; Curran-Rauhut, M; Buechel, HM; Dowling, AL; Brewer, LD; Popovic, J; Thibault, V; Kraner, SD; Chen, KC; Blalock, EM
      PloS one  7  e40128  2012

      Show Abstract
      22792227 22792227
      Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in skeletal muscle without sustained increases in kinase phosphorylation.
      Schweitzer, GG; Arias, EB; Cartee, GD
      Journal of applied physiology (Bethesda, Md. : 1985)  113  1852-61  2012

      Show Abstract
      22936728 22936728
      Increased expression of serum- and glucocorticoid-regulated kinase-1 in the duodenal mucosa of children with coeliac disease.
      Szebeni B, Vannay A, Sziksz E, Prókai A, Cseh A, Veres G, Dezsofi A, Gyorffy H, Szabó IR, Arató A
      J Pediatr Gastroenterol Nutr  50  147-53.  2010

      Show Abstract
      19966577 19966577
      mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1).
      Juan M García-Martínez,Dario R Alessi
      The Biochemical journal  416  2008

      Show Abstract
      18925875 18925875
      Acute activation of NHE3 by dexamethasone correlates with activation of SGK1 and requires a functional glucocorticoid receptor.
      Wang, D; Zhang, H; Lang, F; Yun, CC
      American journal of physiology. Cell physiology  292  C396-404  2007

      Show Abstract
      Western Blotting16971495 16971495
      SGK protein kinase facilitates the expression of long-term potentiation in hippocampal neurons
      Ma, Yun L, et al
      Learn Mem, 13:114-8 ()  2006

      16585788 16585788
      Up-regulation of glucocorticoid-regulated genes in a mouse model of Rett syndrome.
      Nuber, UA; Kriaucionis, S; Roloff, TC; Guy, J; Selfridge, J; Steinhoff, C; Schulz, R; Lipkowitz, B; Ropers, HH; Holmes, MC; Bird, A
      Human molecular genetics  14  2247-56  2005

      Show Abstract
      Western Blotting16002417 16002417
      Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo
      Lizcano, J. M., et al
      J Biol Chem, 277:27839-49 (2002)  2002

      Immunoblotting (Western)12023960 12023960
      Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase
      Kobayashi, T., et al
      Biochem J, 344 Pt 1:189-97 (1999)  1999

      Protein Kinase/Phosphatase Assays10548550 10548550

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies