04-1124 | Anti-SIRT2 (CT) Antibody, clone EP1668Y, rabbit monoclonal

04-1124
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, ICC, FCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1124
      DescriptionAnti-SIRT2 (CT) Antibody, clone EP1668Y, rabbit monoclonal
      Alternate Names
      • SIR2-like protein 2
      • silencing information regulator 2-like
      • silent information regulator 2
      • sir2-related protein type 2
      • sirtuin (silent mating type information regulation 2
        homolog) 2 (S. cerevisiae)
      • sirtuin (silent mating type information regulation 2,
        S.cerevisiae, homolog) 2
      • sirtuin 2
      • sirtuin type 2
      Background InformationSirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.
      References
      Product Information
      FormatUnpurified
      Control
      • SKBR-3 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected SIRT2 in PC12 cells.
      Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.
      Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues on the C-terminus of human SIRT2.
      EpitopeC-terminus
      CloneEP1668Y
      HostRabbit
      SpecificityThis antibody recognizes SIRT2 at and around the C-terminus, all three isoforms.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.
      Gene Symbol
      • EC 3.5.1.-
      • SIR2
      • SIR2-like
      • SIR2L
      • SIR2L2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.
      CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.
      COFACTOR: Binds 1 zinc ion per subunit.
      ENZYME REGULATION: Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide.
      SUBUNIT STRUCTURE: Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton.
      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules.
      TISSUE SPECIFICITY: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
      DEVELOPMENTAL STAGE: Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
      PTM: Phosphorylated at the G2/M transition of the cell cycle.
      SEQUENCE SIMILARITIES: Belongs to the sirtuin family.
      Contains 1 deacetylase sirtuin-type domain.
      SEQUENCE CAUTION: The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.
      Molecular Weight~36-43 kDa (detects all 3 isoforms)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on SKBR-3 cell lysates.
      Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect SIRT2 in SKBR-3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal RABBIT MONOCLONAL
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal - 2197260 2197260
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal - 2326443 2326443
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal - 2343595 2343595
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal - NG1842835 NG1842835
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal - NG1932158 NG1932158
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal - NRG1632695 NRG1632695
      Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal - NRG1635533 NRG1635533

      References

      Reference overviewApplicationPub Med ID
      Profiling deacetylase activities in cell lysates with peptide arrays and SAMDI mass spectrometry.
      Kuo, HY; DeLuca, TA; Miller, WM; Mrksich, M
      Analytical chemistry  85  10635-42  2013

      Show Abstract
      Western Blotting24088168 24088168

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies