05-1426 | Anti-SLP-76 Antibody, clone AS55

05-1426
100 µg  
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      Overview

      Replacement Information
      Replacement Information05-1426 is a recommended replacement for MAB3756

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1426
      ReplacesMAB3756
      DescriptionAnti-SLP-76 Antibody, clone AS55
      Alternate Names
      • 76 kDa tyrosine phosphoprotein 2
      • Human 76 kDa tyrosine phosphoprotein SLP-76 mRNA, complete cds
      • Lymphocyte cytosolic protein 2
      • SH2 domain-containing leukocyte protein of 76 kDa
      • SLP-76 tyrosine phosphoprotein
      • lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
      Background InformationDetects SLP-76 (SH2 domain containing Leukocyte Protein) of 76 kDa, a hematopoietic cell-specific adapter protein which intereacts with other signaling molecules through amino-terminal phosphotyrosine residues, a central proline-rich region and a carboxyl-terminal Src homology 2 domain (SH2).
      References
      Product Information
      FormatPurified
      Control
      • RAW264 cell lysate
      PresentationPurified mouse monoclonal IgG2a 0.2 mm sterile filtered solution in phosphate buffered saline with 0.1% sodium azide.
      Applications
      ApplicationDetect SLP-76 using this Anti-SLP-76 Antibody, clone AS55 validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: A previous lot of this antibody was used in IP.
      Optimal working dilutions must be determined by end user.

      Biological Information
      ImmunogenMonoclonal antibody from BALB/c-derived hybridoma
      CloneAS55
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes human SH2 (Src homology 2) domain-Leukocyte Protein of 76 kDa.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and mouse. Expected to react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function
      Gene Symbol
      • LCP2
      • SLP-76
      • SLP76
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13094 # Involved in T-cell antigen receptor mediated signaling.
      SIZE: 533 amino acids; 60188 Da
      SUBUNIT: Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.
      SUBCELLULAR LOCATION: Cytoplasm (Probable).
      TISSUE SPECIFICITY: Highly expressed in spleen, thymus, and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.
      DOMAIN:SwissProt: Q13094 The SH2 domain mediates interaction with SHB.
      PTM: Phosphorylated after T-cell receptor activation by ZAP-70.
      SIMILARITY: Contains 1 SH2 domain.
      Molecular WeightDetects SLP-76 (SH2 domain containing Leukocyte Protein) of 76 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on RAW 264 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected SLP-76 on 10 μg of RAW 264 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SLP 76, clone AS55 - 2378770 2378770
      Anti-SLP 76, clone AS55 - 2449191 2449191
      Anti-SLP 76, clone AS55 - 2007352 2007352
      Anti-SLP 76, clone AS55 - 2377055 2377055
      Anti-SLP 76, clone AS55 - NG1578602 NG1578602
      Anti-SLP 76, clone AS55 - NG1583003 NG1583003
      Anti-SLP 76, clone AS55 - NG1734238 NG1734238
      Anti-SLP 76, clone AS55 -2569793 2569793
      Anti-SLP 76, clone AS55 -2584417 2584417
      Anti-SLP 76, clone AS55 -2584607 2584607