07-710 | Anti-SMC2/hCAP-E Antibody

07-710
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-710
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SMC2/hCAP-E Antibody
      OverviewSMC2 and SMC4 from the active ATPase subunit of Condensin I and Condensin II complexes that regulate chromosome compaction.
      References
      Product Information
      FormatSerum
      Applications
      ApplicationAnti-SMC2/hCAP-E Antibody is a high quality Rabbit Polyclonal Antibody for the detection of SMC2/hCAP-E & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1184-1197 (C-KSKAKPPKGAHVEV) of human SMC2/hCAP-E (Structural Maintenance of Chromosome 2/ Chromosome-Associated Protein E) with an N-terminal cysteine added for conjugation purposes.
      HostRabbit
      SpecificitySMC2/hCAP-E
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMC2
      • SMC2L1
      • hCAP-E
      • FLJ10093
      • CAPE
      • CAP-E
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O95347 # Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
      SIZE: 1197 amino acids; 135781 Da
      SUBUNIT: Forms an heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
      DOMAIN: SwissProt: O95347 The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer (By similarity).
      SIMILARITY: Belongs to the SMC family. SMC2 subfamily.
      Molecular Weight137kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SMC2/hCAP-E (rabbit antiserum) 2982352
      Anti-SMC2/hCAP-E - 30447 30447
      Anti-SMC2/hCAP-E -2608404 2608404

      References

      Reference overviewApplicationPub Med ID
      Human SMC2 protein, a core subunit of human condensin complex, is a novel transcriptional target of the WNT signaling pathway and a new therapeutic target.
      Dávalos, V; Súarez-López, L; Castaño, J; Messent, A; Abasolo, I; Fernandez, Y; Guerra-Moreno, A; Espín, E; Armengol, M; Musulen, E; Ariza, A; Sayós, J; Arango, D; Schwartz, S
      The Journal of biological chemistry  287  43472-81  2012

      Show Abstract
      Western Blotting23095742 23095742
      Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners.
      Schleiffer, Alexander, et al.
      Mol. Cell, 11: 571-5 (2003)  2003

      Show Abstract
      12667442 12667442
      Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
      Ono, Takao, et al.
      Cell, 115: 109-21 (2003)  2003

      Show Abstract
      14532007 14532007
      Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A
      Yeong, F. M., et al
      Curr Biol, 13:2058-64 (2003)  2003

      Immunoprecipitation14653995 14653995
      Classification, subtype discovery, and prediction of outcome in pediatric acute lymphoblastic leukemia by gene expression profiling.
      Yeoh, Eng-Juh, et al.
      Cancer Cell, 1: 133-43 (2002)  2002

      Show Abstract
      12086872 12086872
      Chromosome condensation by a human condensin complex in Xenopus egg extracts.
      Kimura, K, et al.
      J. Biol. Chem., 276: 5417-20 (2001)  2001

      Show Abstract
      11136719 11136719
      A human condensin complex containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation.
      Schmiesing, J A, et al.
      Mol. Cell. Biol., 20: 6996-7006 (2000)  2000

      Show Abstract
      10958694 10958694
      Identification of two distinct human SMC protein complexes involved in mitotic chromosome dynamics.
      Schmiesing, J A, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 12906-11 (1998)  1998

      Show Abstract
      9789013 9789013

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