07-249 | Anti-SMURF2 Antibody

07-249
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-249
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SMURF2 Antibody
      References
      Product Information
      FormatSerum
      Presentationantiserum containing 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect SMURF2 with Anti-SMURF2 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 161-174 (C-DGWEERRTASGRIQ) of human SMURF 2
      HostRabbit
      SpecificitySMURF 2
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMURF2
      • DKFZp686F0270
      • MGC138150
      • hSMURF2
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9HAU4 # E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
      SIZE: 748 amino acids; 86196 Da
      SUBUNIT: Interacts with SMAD1, SMAD2, SMAD3, SMAD6 and SMAD7 but not SMAD4.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Cytoplasmic in the presence of SMAD7.
      TISSUE SPECIFICITY: Widely expressed.
      DOMAIN:SwissProt: Q9HAU4 The second and third WW domains are responsible for interaction with R-SMAD (SMAD1, SMAD2 and SMAD3).
      SIMILARITY: Contains 1 C2 domain. & Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. & Contains 3 WW domains.
      Molecular Weight86kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from HepG2 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SMURF2 - 22041 22041

      References

      Reference overviewApplicationPub Med ID
      Downregulation of Smurf2, a tumor-suppressive ubiquitin ligase, in triple-negative breast cancers: involvement of the RB-microRNA axis.
      Liu, X; Gu, X; Sun, L; Flowers, AB; Rademaker, AW; Zhou, Y; Kiyokawa, H
      BMC cancer  14  57  2014

      Show Abstract
      Western Blotting24485087 24485087
      The E3 ubiquitin ligase SMAD ubiquitination regulatory factor 2 negatively regulates Krüppel-like factor 5 protein.
      Du, JX; Hagos, EG; Nandan, MO; Bialkowska, AB; Yu, B; Yang, VW
      The Journal of biological chemistry  286  40354-64  2011

      Show Abstract
      Western Blotting21953463 21953463
      Regulation of TGF-beta signalling by Fbxo11, the gene mutated in the Jeff otitis media mouse mutant.
      Tateossian, H; Hardisty-Hughes, RE; Morse, S; Romero, MR; Hilton, H; Dean, C; Brown, SD
      PathoGenetics  2  5  2009

      Show Abstract
      19580641 19580641
      Expression of Smad ubiquitin regulatory factor 2 (Smurf2) in rhesus monkey endometrium and placenta during early pregnancy.
      Yang, Q; Lin, HY; Wang, HX; Zhang, H; Zhang, X; Wang, HM; Zhu, C
      The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society  55  453-60  2007

      Show Abstract
      17189523 17189523
      LIM mineralization protein-1 potentiates bone morphogenetic protein responsiveness via a novel interaction with Smurf1 resulting in decreased ubiquitination of Smads.
      Sangadala, S; Boden, SD; Viggeswarapu, M; Liu, Y; Titus, L
      The Journal of biological chemistry  281  17212-9  2006

      Show Abstract
      Western Blotting16611643 16611643

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies