05-522 | Anti-SRC-1 Antibody, clone 1135

05-522
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-522
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SRC-1 Antibody, clone 1135
      Alternate Names
      • NCoA-1
      • SRC1
      • SRC-1
      • KAT13A
      • RIP160
      • MGC129719
      • MGC129720
      Background InformationSteroid Receptor Coactivator-1 (SRC-1) is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain. SRC-1 is also known as Nuclear receptor coactivator 1 (NCoA-1).
      References
      Product Information
      FormatPurified
      Control
      • Cos-1 cell lysate
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-SRC-1 Antibody, clone 1135 is a Mouse Monoclonal Antibody for the detection of SRC-1 & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion-protein containing amino acids 477-947 of human SRC-1 (Steroid Receptor Coactivator-1)
      Cloneclone 1135
      HostMouse
      SpecificitySRC-1 isoforms
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • NCOA1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da
      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight160kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Cos-1 cell lysate or HeLa Nuclear Extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SRC-1, clone 1135 2480577
      Anti-SRC-1, clone 1135 - 18883 18883
      Anti-SRC-1, clone 1135 - 20690 20690
      Anti-SRC-1, clone 1135 - 21604 21604
      Anti-SRC-1, clone 1135 - 22759 22759
      Anti-SRC-1, clone 1135 - 28736 28736
      Anti-SRC-1, clone 1135 - DAM1831388 DAM1831388
      Anti-SRC-1, clone 1135 Monoclonal Antibody 2976284

      References

      Reference overviewApplicationSpeciesPub Med ID
      Progesterone receptor and SRC-1 participate in the regulation of VEGF, EGFR and Cyclin D1 expression in human astrocytoma cell lines.
      Olivia Tania Hernández-Hernández,Tania Karina González-García,Ignacio Camacho-Arroyo
      The Journal of steroid biochemistry and molecular biology  132  2012

      Show Abstract
      22542550 22542550
      Estradiol increases cell growth in human astrocytoma cell lines through ERα activation and its interaction with SRC-1 and SRC-3 coactivators.
      Aliesha González-Arenas,Valeria Hansberg-Pastor,Olivia Tania Hernández-Hernández,Tania Karina González-García,Joshua Henderson-Villalpando,Diana Lemus-Hernández,Aglaé Cruz-Barrios,Mariana Rivas-Suárez,Ignacio Camacho-Arroyo
      Biochimica et biophysica acta  1823  2012

      Show Abstract
      22142990 22142990
      Systems genetics analyses predict a transcription role for P2P-R: molecular confirmation that P2P-R is a transcriptional co-repressor.
      Peidis, P; Giannakouros, T; Burow, ME; Williams, RW; Scott, RE
      BMC systems biology  4  14  2010

      Show Abstract Full Text Article
      20184719 20184719
      Gonadotropin-releasing hormone-mediated phosphorylation of estrogen receptor-alpha contributes to fosB expression in mouse gonadotrophs.
      Chen, J; An, BS; Cheng, L; Hammond, GL; Leung, PC
      Endocrinology  150  4583-93  2009

      Show Abstract
      Western Blotting19574399 19574399
      Regulation of estrogen receptor-mediated long range transcription via evolutionarily conserved distal response elements.
      Pan, YF; Wansa, KD; Liu, MH; Zhao, B; Hong, SZ; Tan, PY; Lim, KS; Bourque, G; Liu, ET; Cheung, E
      The Journal of biological chemistry  283  32977-88  2008

      Show Abstract
      18728018 18728018
      Oxysterol and diabetes activate STAT3 and control endothelial expression of profilin-1 via OSBP1.
      Romeo, GR; Kazlauskas, A
      The Journal of biological chemistry  283  9595-605  2008

      Show Abstract
      Western BlottingRat18230613 18230613
      Estrogen receptor beta isoform-specific induction of transforming growth factor beta-inducible early gene-1 in human osteoblast cells: an essential role for the activation function 1 domain.
      Hawse, JR; Subramaniam, M; Monroe, DG; Hemmingsen, AH; Ingle, JN; Khosla, S; Oursler, MJ; Spelsberg, TC
      Molecular endocrinology (Baltimore, Md.)  22  1579-95  2008

      Show Abstract
      18483178 18483178
      Progressive loss of estrogen receptor alpha cofactor recruitment in endocrine resistance.
      Naughton, C; MacLeod, K; Kuske, B; Clarke, R; Cameron, DA; Langdon, SP
      Molecular endocrinology (Baltimore, Md.)  21  2615-26  2007

      Show Abstract
      Western Blotting17666584 17666584
      Estrogen receptor isoform-specific regulation of the retinoblastoma-binding protein 1 (RBBP1) gene: roles of AF1 and enhancer elements.
      Monroe, DG; Secreto, FJ; Hawse, JR; Subramaniam, M; Khosla, S; Spelsberg, TC
      The Journal of biological chemistry  281  28596-604  2006

      Show Abstract
      16873370 16873370
      Steroid receptor coactivator-3 is required for progesterone receptor trans-activation of target genes in response to gonadotropin-releasing hormone treatment of pituitary cells.
      An, BS; Selva, DM; Hammond, GL; Rivero-Muller, A; Rahman, N; Leung, PC
      The Journal of biological chemistry  281  20817-24  2006

      Show Abstract
      16728408 16728408

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies