04-479 | Anti-SREBP-2 Antibody, clone 2545

04-479
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, WBMAscitesMonoclonal Antibody
      Description
      Catalogue Number04-479
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SREBP-2 Antibody, clone 2545
      Alternate Names
      • SREBP2
      • sterol regulatory element binding transcription factor 2
      References
      Product Information
      FormatAscites
      Presentation200 μL of mouse ascites of monoclonal IgG1 with 0.1% sodium azide.
      Applications
      ApplicationUse Anti-SREBP-2 Antibody, clone 2545 (Mouse Monoclonal Antibody) validated in ELISA, WB to detect SREBP-2 also known as sterol regulatory element binding transcription factor 2.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenRecombinant GST-tagged human SREBP-2 protein encompassing amino acids 48-403.
      CloneClone 2545
      HostMouse
      SpecificityReacts with both the precursor and mature forms of SREBP-2,
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.
      Gene Symbol
      • SREBF2
      • OTTHUMP00000028740
      • SREBP-2
      • SREBP2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q12772 # Transcriptional activator that binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
      SIZE: 1141 amino acids; 123688 Da
      SUBUNIT: Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein.
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
      TISSUE SPECIFICITY: Ubiquitously expressed in adult and fetal tissues.
      PTM: Under sterol-depleted conditions, SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage occurs within the luminal loop and is directly regulated by sterols. The second cleavage by site-2 protease occurs within the first transmembrane stretch and liberates the soluble transcription factor. Cleavage by the cysteine proteases, caspase- 3 and caspase-7, is induced during apoptosis, independent of sterol levels.
      SIMILARITY: SwissProt: Q12772 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      MISCELLANEOUS: SREBPs have to be in a complex with the cleavage- activating protein (SCAP) to move to the Golgi and be cleaved by site-1 protease.
      Molecular Weight124 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 (Mouse Monoclonal Ascites) - 2195986 2195986
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 (Mouse Monoclonal Ascites) - 2273975 2273975
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 (Mouse Monoclonal Ascites) - DAM1823474 DAM1823474
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 - 0611044481 0611044481
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 - 0703056203 0703056203
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 - 2011239 2011239
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 - 2068201 2068201
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 - 2530010 2530010
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 - DAM1574339 DAM1574339
      Anti-Sterol Regulatory Element Binding Protein 2 (SREBP-2), clone 2545 - DAM1811100 DAM1811100

      References

      Reference overviewPub Med ID
      The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols
      Janowski, B A, et al
      J Biol Chem, 276:45408-16 (2001)  2001

      11577112 11577112