04-469 | Anti-SREBP1 Antibody, clone 2121

04-469
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-469
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SREBP1 Antibody, clone 2121
      Alternate Names
      • SREBF1
      • sterol regulatory element binding transcription factor 1
      References
      Product Information
      FormatPurified
      Presentation100 μL (1 mg/mL) of protein A purified mouse monoclonal IgG1 in PBS with 0.1% sodium azide.
      Applications
      ApplicationAnti-SREBP1 Antibody, clone 2121 is a Mouse Monoclonal Antibody for detection of SREBP1 also known as sterol regulatory element binding transcription factor 1 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant His-tagged human SREBP-1a protein encompassing amino acids 301-407.
      Clone2121
      HostMouse
      SpecificityReacts with the basic helix-loop-helix leucine zipper (bHLH-ZIP) of SREBP.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • SREBF1
      • SREBP1
      • SREBP-1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P36956 # Transcriptional activator that binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity, binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the transcription of genes for sterol biosynthesis and the LDL receptor gene.
      SIZE: 1147 amino acids; 121645 Da
      SUBUNIT: Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein.
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
      TISSUE SPECIFICITY: Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
      PTM: Under sterol-depleted conditions, SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage occurs within the luminal loop and is directly regulated by sterols. The second cleavage by site-2 protease occurs within the first transmembrane stretch and liberates the soluble transcription factor. Cleavage by the cysteine proteases, caspase- 3 and caspase-7, is induced during apoptosis, independent of sterol levels.
      SIMILARITY: SwissProt: P36956 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      MISCELLANEOUS: SREBPs have to be in a complex with the cleavage- activating protein (SCAP) to move to the Golgi and be cleaved by site-1 protease.
      Molecular Weight126 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti SREBP-1a, clone 2121 (Protein A purified Mouse Monoclonal) Q2922311
      Anti-Sterol Regulatory Element Binding Protein 1 (SREBP1), clone 2121 - 0611044480 0611044480
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 (Protein A purified Mouse Monoclonal) - 2392289 2392289
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 (Protein A purified Mouse Monoclonal) 2918911
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 (Protein A purified Mouse Monoclonal) - 2036866 2036866
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 (Protein A purified Mouse Monoclonal) - 2195983 2195983
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 (Protein A purified Mouse Monoclonal) - DAM1413961 DAM1413961
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 (Protein A purified Mouse Monoclonal) - DAM1474310 DAM1474310
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 - DAM1650336 DAM1650336
      Anti-Sterol Regulatory Element Binding Protein 1a (SREBP-1a), clone 2121 - DAM1679967 DAM1679967

      References

      Reference overviewPub Med ID
      Phosphorylation and ubiquitination of the transcription factor sterol regulatory element-binding protein-1 in response to DNA binding
      Punga, Tanel, et al
      J Biol Chem, 281:25278-86 (2006)  2006

      16825193 16825193
      Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. II. Purification and characterization
      Wang, X, et al
      J Biol Chem, 268:14497-504 (1993)  1993

      8314806 8314806