06-1364 | Anti-SRp30b (SC35) Antibody

06-1364
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1364
      DescriptionAnti-SRp30b (SC35) Antibody
      Alternate Names
      • serine/arginine-rich splicing factor 2
      • Splicing factor SC35
      • Splicing component, 35 kDa
      • splicing factor, arginine/serine-rich 2
      • SR splicing factor 2
      Background InformationSRp30b, also known as SC35, and Splicing factor, arginine/serine-rich 2 (SFRS2) is an essential splicing factor. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SRp30b is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is not completely characterized. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SRp30b is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. This SR protein is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cells.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-SRp30b (SC35) Antibody is a Rabbit Polyclonal Antibody for detection of SRp30b (SC35) also known as serine/arginine-rich splicing factor 2, Splicing factor SC35 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis:
      A 1:500 dilution of a representative lot of this antibody detected SRp30b in 10 µg of HeLa cell lysate.

      Immunoprecipitation Analysis:
      A representative lot of this antibody was used by an independent laboratory in IP. (Jiang, K., et al. (2009). Endocrinology. 150(5):2087-2097.)
      Biological Information
      ImmunogenLinear peptide corresponding to human SRp30b.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq].
      Gene Symbol
      • SC-35
      • PR264
      • SFRS2
      • SC35
      • SRp30b
      • SFRS2A
      • Protein PR2643
      • SRSF2
      Purification MethodProtein A
      UniProt Number
      UniProt SummaryFUNCTION: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.

      SUBUNIT STRUCTURE: In vitro, self-associates and binds SRSF1/SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SFRS12. Interacts with CCNL1 and CCNL2. Interacts with SRSF2IP/SFRS2IP. Interacts with ZRSR2/U2AF1-RS2.

      SUBCELLULAR LOCATION: Nucleus.

      PTM: Extensively phosphorylated on serine residues in the RS domain.

      SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.

      Contains 1 RRM (RNA recognition motif) domain.
      Molecular Weight~35 kDa observed. Uncharacterized bands may be observed at ~10, 58, and 78 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry Analysis:
      A 1:500 dilution of this antibody detected SRp30b in NIH/3T3 cells.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      04-1550 Anti-SC-35 Antibody, clone 1SC-4F11 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies