04-1100 | Anti-Smad1 Antibody, clone EP565Y, rabbit monoclonal

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, WBRbUnpurifiedMonoclonal Antibody
      Catalogue Number04-1100
      DescriptionAnti-Smad1 Antibody, clone EP565Y, rabbit monoclonal
      Alternate Names
      • MAD, mothers against decapentaplegic homolog 1
      • MAD, mothers against decapentaplegic homolog 1 (Drosophila)
      • Mad-related protein 1
      • Mothers against DPP homolog 1
      • SMAD 1
      • SMAD family member 1
      • SMAD, mothers against DPP homolog 1
      • SMAD, mothers against DPP homolog 1 (Drosophila)
      • Sma- and Mad-related protein 1
      • TGF-beta signaling protein 1
      • Transforming growth factor-beta-signaling protein 1
      • transforming growth factor-beta signaling protein 1
      Background InformationSmad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.
      Product Information
      • HeLa cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationAnti-Smad1 Antibody, clone EP565Y is a Rabbit Monoclonal Antibody for detection of Smad1 also known as MAD-mothers against decapentaplegic-homolog 1 & has been validated in ICC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected Smad1 in HeLa cells.
      Biological Information
      ImmunogenSynthetic peptide from human Smad1.
      SpecificityThis antibody recognizes Smad1.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C.
      elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis,
      morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation.
      This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript
      variants encoding the same protein have been observed.
      Gene Symbol
      • BSP-1
      • BSP1
      • CMT2D
      • GARS
      • HMN5
      • JV4-1
      • JV41
      • MADH1
      • MADR1
      • hSMAD1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).
      SUBUNIT STRUCTURE: Interacts with HGS, NANOG and ZCCHC12 By similarity. May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1. Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
      TISSUE SPECIFICITY:Ubiquitous. Highest expression seen in the heart and skeletal muscle.
      PTM: Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
      SEQUENCE SIMILARITIES: Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
      Molecular Weight~58 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on HeLa cell lysates.
      Western Blot Analysis: A 1:500-2,000 dilution of this antibody was used to detect Smad1 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies