06-920 | Anti-Smad3 Antibody

06-920
400 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-920
      Replaces04-1035
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Smad3 Antibody
      Background InformationMembers of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
      References
      Product Information
      FormatPurified
      Control
      • HepG2 cell lysate
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect Smad3 with Anti-Smad3 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide [C-NLSPNPMSPAHNNLD-amide] corresponding to amino acids 206-220 of human Smad3
      HostRabbit
      SpecificitySmad3
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMAD3
      • MADH3
      • JV15-2
      • DKFZp686J10186
      • hSMAD3
      • hMAD-3
      • HSPC193
      • MGC60396
      • HsT17436
      • Mad3
      • DKFZP586N0721
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P84022 # Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD) (By similarity).
      SIZE: 425 amino acids; 48081 Da
      SUBUNIT: Interacts with HGS. Interacts with NEDD4L in response to TGF-beta (By similarity). Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co- SMAD SMAD4. Interacts with JUN/FOS, vitamin D receptor, homeobox protein TGIF and TGIF2, PEBP2-alpha C subunit, CREB-binding protein (CBP), p300, SKI, SNON, ATF2, SMURF2, AIP1, DACH1 and TGFB1I1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Found in a complex with SMAD2 and TRIM33 upon addition of TGF-beta. Interacts with SMAD2 and TRIM33. Found in a complex with SMAD3, Ran and XPO4. Interacts with XPO4.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity).
      DOMAIN: SwissProt: P84022 The MH2 domain is sufficient to carry protein nuclear export.
      PTM: Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.DISEASE:SwissProt: P84022 # Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].
      SIMILARITY: Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain.
      Molecular Weight50kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from HepG2 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size400 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Smad3 - 19649 19649
      Anti-Smad3 - 23222 23222
      Anti-Smad3 - 26524 26524
      Anti-Smad3 - 31640 31640
      Anti-Smad3 -2522077 2522077
      Anti-Smad3 -2607876 2607876
      Anti-Smad3 -2776742 2776742
      Anti-Smad3 -2801966 2801966

      References

      Reference overviewApplicationPub Med ID
      Smad3 mediates ANG II-induced hypertensive kidney disease in mice.
      Liu, Z; Huang, XR; Lan, HY
      American journal of physiology. Renal physiology  302  F986-97  2012

      Show Abstract
      22237801 22237801
      SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression.
      Ding, Z; Wu, CJ; Chu, GC; Xiao, Y; Ho, D; Zhang, J; Perry, SR; Labrot, ES; Wu, X; Lis, R; Hoshida, Y; Hiller, D; Hu, B; Jiang, S; Zheng, H; Stegh, AH; Scott, KL; Signoretti, S; Bardeesy, N; Wang, YA; Hill, DE; Golub, TR; Stampfer, MJ; Wong, WH; Loda, M; Mucci, L; Chin, L; DePinho, RA
      Nature  470  269-73  2011

      Show Abstract
      21289624 21289624
      A positive role for Myc in TGFbeta-induced Snail transcription and epithelial-to-mesenchymal transition.
      Smith, AP; Verrecchia, A; Fagà, G; Doni, M; Perna, D; Martinato, F; Guccione, E; Amati, B
      Oncogene  28  422-30  2009

      Show Abstract
      18978814 18978814
      Regulation of TGF-beta signalling by Fbxo11, the gene mutated in the Jeff otitis media mouse mutant.
      Tateossian, H; Hardisty-Hughes, RE; Morse, S; Romero, MR; Hilton, H; Dean, C; Brown, SD
      PathoGenetics  2  5  2009

      Show Abstract
      19580641 19580641
      Matrix metalloproteinase gelatinase B (MMP-9) coordinates and effects epithelial regeneration
      Mohan, R., et al
      J Biol Chem, 277:2065-72 (2002)  2002

      Immunoblotting (Western)11689563 11689563
      Smad4/DPC4 and Smad3 mediate transforming growth factor-beta (TGF-beta) signaling through direct binding to a novel TGF-beta-responsive element in the human plasminogen activator inhibitor-1 promoter.
      Song, C Z, et al.
      J. Biol. Chem., 273: 29287-90 (1998)  1998

      Show Abstract
      9792626 9792626

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies