07-645 | Anti-Sp1 Antibody

07-645
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, REMSA, IF, IP, WB, ChIPRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-645
      ReplacesMABE550
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Sp1 Antibody
      Alternate Names
      • Sp1 transcription factor
      • specificity protein 1
      Background InformationSp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation:
      4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
      NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com

      Chromatin Immunoprecipitation:
      Reported by an independent laboratory.

      Immunofluorescence:
      Reported by an independent laboratory.

      Electrophoretic Mobility Shift Assay (EMSA):
      1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
      Biological Information
      ImmunogenFull length human Sp1 protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Sp1, Mr 105 kDa and 95 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and mouse. Expected to cross react with rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TSFP1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.
      SIZE: 785 amino acids; 80693 Da
      SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: O-glycosylated; contains N-acetylglucosamine side chains.
      SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
      Molecular WeightVaries
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sp1 3018769
      Anti-Sp1 2475744
      Anti-Sp1 (rabbit polyclonal IgG) - JBC1395555 JBC1395555
      Anti-Sp1 (rabbit polyclonal) Polyclonal Antibody Q2909958
      Anti-Sp1 - 2446853 2446853
      Anti-Sp1 - 0610043180 0610043180
      Anti-Sp1 - 0704057821 0704057821
      Anti-Sp1 - 1984846 1984846
      Anti-Sp1 - 2073142 2073142
      Anti-Sp1 - 2207273 2207273

      References

      Reference overviewApplicationPub Med ID
      CR6-interacting factor 1 is a key regulator in Aβ-induced mitochondrial disruption and pathogenesis of Alzheimer's disease.
      Byun, J; Son, SM; Cha, MY; Shong, M; Hwang, YJ; Kim, Y; Ryu, H; Moon, M; Kim, KS; Mook-Jung, I
      Cell death and differentiation  22  959-73  2015

      Show Abstract
      25361083 25361083
      An Sp1 Modulated Regulatory Region Unique to Higher Primates Regulates Human Androgen Receptor Promoter Activity in Prostate Cancer Cells.
      Hay, CW; Hunter, I; MacKenzie, A; McEwan, IJ
      PloS one  10  e0139990  2015

      Show Abstract
      26448047 26448047
      Cezanne regulates E2F1-dependent HIF2α expression.
      Moniz, S; Bandarra, D; Biddlestone, J; Campbell, KJ; Komander, D; Bremm, A; Rocha, S
      Journal of cell science  128  3082-93  2015

      Show Abstract
      26148512 26148512
      Erythroid induction of K562 cells treated with mithramycin is associated with inhibition of raptor gene transcription and mammalian target of rapamycin complex 1 (mTORC1) functions.
      Finotti, A; Bianchi, N; Fabbri, E; Borgatti, M; Breveglieri, G; Gasparello, J; Gambari, R
      Pharmacological research  91  57-68  2015

      Show Abstract
      25478892 25478892
      HDAC1 and Klf4 interplay critically regulates human myeloid leukemia cell proliferation.
      Huang, Y; Chen, J; Lu, C; Han, J; Wang, G; Song, C; Zhu, S; Wang, C; Li, G; Kang, J; Wang, J
      Cell death & disease  5  e1491  2014

      Show Abstract
      25341045 25341045
      Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter.
      Liu, M; Wang, X; Peng, Y; Shen, S; Li, G
      BMC molecular biology  15  14  2014

      Show Abstract
      25029911 25029911
      Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma.
      Miyata, K; Yotsumoto, F; Nam, SO; Odawara, T; Manabe, S; Ishikawa, T; Itamochi, H; Kigawa, J; Takada, S; Asahara, H; Kuroki, M; Miyamoto, S
      Cancer medicine  3  1159-69  2014

      Show Abstract
      Western Blotting25060396 25060396
      Down-regulation of Sp1 suppresses cell proliferation, clonogenicity and the expressions of stem cell markers in nasopharyngeal carcinoma.
      Zhang, JP; Zhang, H; Wang, HB; Li, YX; Liu, GH; Xing, S; Li, MZ; Zeng, MS
      Journal of translational medicine  12  222  2014

      Show Abstract
      25099028 25099028
      Regulatory mechanism of endothelin receptor B in the cerebral arteries after focal cerebral ischemia.
      Grell, AS; Thigarajah, R; Edvinsson, L; Samraj, AK
      PloS one  9  e113624  2014

      Show Abstract
      Immunohistochemistry25479176 25479176
      Loss of Jak2 Impairs Endothelial Function by Attenuating Raf-1/MEK1/Sp-1 Signaling Along with Altered eNOS Activities.
      Yang, Ping, et al.
      Am. J. Pathol., 183: 617-25 (2013)  2013

      Show Abstract
      23747947 23747947

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies