07-107 | Anti-Sp3 Antibody

07-107
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbEMSA, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-107
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Sp3 Antibody
      Background InformationThe sequence-specific transcription factor Sp3 binds GC and GT box sequences in the promoters of a variety of genes. The Sp3 transcription factor contains three zinc finger domains that determine sequence specificity of DNA binding. This transcription factor is thought to regulate nucleoside, nucleotide and nucleic acid metabolism and many other “housekeeping” genes. Sp3 can be modified by sumoylation by Ubc9 and has been shown to interact with other transcription factors and histonbe deacetylases. Sp3 has been reported to act as a transcriptional enhancer and repressor of Sp1 activity in a cell- and context-dependent manner.
      References
      Product Information
      FormatPurified
      Control
      • Raji cell nuclear extract
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect Sp3 with Anti-Sp3 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in EMSA, IP & WB.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenFusion protein corresponding to amino acid residues 239-653 of human Sp3
      HostRabbit
      SpecificitySp3 isoforms
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted.
      Gene Symbol
      • SP3
      • DKFZp686O1631
      • SPR-2
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q02447 # Transcriptional factor that can act as an activator or repressor, probably in a isoform-specific manner. Binds to GT and GC boxes promoters elements.
      SIZE: 781 amino acids; 81925 Da
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitously expressed.
      SIMILARITY: SwissProt: Q02447 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
      Molecular Weight58kDa, 60kDa and 110kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Raji nuclear extract or HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sp3 (rabbit polyclonal IgG) - 2137073 2137073
      Anti-Sp3 (rabbit polyclonal IgG) - 2197509 2197509
      Anti-Sp3 (rabbit polyclonal IgG) - 2283543 2283543
      Anti-Sp3 - 20101 20101
      Anti-Sp3 - 24702 24702
      Anti-Sp3 - 28847 28847
      Anti-Sp3 - 31966 31966
      Anti-Sp3 - JBC1872192 JBC1872192

      References

      Reference overviewApplicationPub Med ID
      Sp1 trans-activates and is required for maximal aldosterone induction of the αENaC gene in collecting duct cells.
      Yu, Z; Kong, Q; Kone, BC
      American journal of physiology. Renal physiology  305  F653-62  2013

      Show Abstract
      23804453 23804453
      Constitutive expression of the brg1 gene requires GC-boxes near to the transcriptional start site.
      Itoh T, Miyake K, Yamaguchi T, Tsuge M, Kaneoka H, Iijima S
      J Biochem  149  301-9. Epub 2010 Dec 10.  2011

      Show Abstract
      21149256 21149256
      Interleukin-1beta induces increased transcriptional activation of the transforming growth factor-beta-activating integrin subunit beta8 through altering chromatin architecture.
      Markovics, JA; Araya, J; Cambier, S; Somanath, S; Gline, S; Jablons, D; Hill, A; Wolters, PJ; Nishimura, SL
      The Journal of biological chemistry  286  36864-74  2011

      Show Abstract
      21878622 21878622
      Transcription of the transforming growth factor beta activating integrin beta8 subunit is regulated by SP3, AP-1, and the p38 pathway.
      Markovics, JA; Araya, J; Cambier, S; Jablons, D; Hill, A; Wolters, PJ; Nishimura, SL
      The Journal of biological chemistry  285  24695-706  2010

      Show Abstract Full Text Article
      20519498 20519498
      Corticosteroids induce cyclooxygenase 1 expression in cardiomyocytes: role of glucocorticoid receptor and Sp3 transcription factor.
      Sun, H; Sheveleva, E; Chen, QM
      Molecular endocrinology (Baltimore, Md.)  22  2076-84  2008

      Show Abstract Full Text Article
      18599619 18599619
      Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma.
      Stegmaier, K; Wong, JS; Ross, KN; Chow, KT; Peck, D; Wright, RD; Lessnick, SL; Kung, AL; Golub, TR
      PLoS medicine  4  e122  2007

      Show Abstract
      17425403 17425403
      Activation of the RNA-dependent protein kinase PKR promoter in the absence of interferon is dependent upon Sp proteins.
      Das, S; Ward, SV; Tacke, RS; Suske, G; Samuel, CE
      The Journal of biological chemistry  281  3244-53  2006

      Show Abstract
      16339759 16339759
      Retinoic acid-induced chromatin remodeling of mouse kappa opioid receptor gene.
      Park, SW; Huq, MD; Loh, HH; Wei, LN
      The Journal of neuroscience : the official journal of the Society for Neuroscience  25  3350-7  2005

      Show Abstract
      15800190 15800190
      The binding of the ubiquitous transcription factor Sp1 at the locus control region represses the expression of beta-like globin genes.
      Feng, D; Kan, YW
      Proceedings of the National Academy of Sciences of the United States of America  102  9896-900  2005

      Show Abstract
      15998736 15998736
      Sp1 is involved in Akt-mediated induction of VEGF expression through an HIF-1-independent mechanism.
      Pore, N; Liu, S; Shu, HK; Li, B; Haas-Kogan, D; Stokoe, D; Milanini-Mongiat, J; Pages, G; O'Rourke, DM; Bernhard, E; Maity, A
      Molecular biology of the cell  15  4841-53  2004

      Show Abstract
      Western Blotting15342781 15342781