05-184-AF488 | Anti-Src Antibody, clone GD11, Alexa Fluor® 488 conjugate

05-184-AF488
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, R, M, H, MiICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-184-AF488
      DescriptionAnti-Src Antibody, clone GD11, Alexa Fluor® 488 conjugate
      Alternate Names
      • Tyrosine-protein kinase transforming protein Src
      • pp60v-src
      • p60-Src
      • v-Src
      • Proto-oncogene tyrosine-protein kinase Src
      • p60-Src
      • pp60c-src
      • Proto-oncogene c-Src
      Background InformationTyrosine-protein kinase transforming protein Src (EC 2.7.10.2; UniProt P00526; also known as p60-Src, pp60v-src, v-Src) is encoded by the V-SRC gene (Gene ID 1491925) of Rous sarcoma virus strain Prague C (RSV-PrC).
      Rous sarcoma virus (RSV) V-SRC gene is the first retroviral oncogene discovered. V-SRC encodes the v-Src tyrosine kinase that transforms infected host cells into uncontrolled mitosis, providing abundant cells for fresh infection. The non-oncogenic chicken c-Src (cellular Src) gene structurally related to v-Src was discovered later in 1979 by J. Michael Bishop and Harold E. Varmus. It is believed that RSV originally took up the chicken proto-oncogene SRC (Gene ID 396442; UniProt P00523) and incorporated into its genome in the mutated concogene form. c-Src contains six functional regions, Src homology 4 (SH4) domain, unique region, SH3 domain, SH2 domain, catalytic domain, and short regulatory tail. In its inactive state, Src is phosphorylated at Tyr527. The pTyr527 interacts with the SH2 domain which helps the SH3 domain interact with the linker domain and consequently keeps the inactive unit tightly bound. Dephosphorylation of pTyr527 destabilizes the inactive conformation, opening up the SH3, SH2 and the kinase domains, leading to Tyr416 autophosphorylation and c-Src activation.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Applications
      ApplicationAnti-Src Antibody, clone GD11, Alexa Fluor® 488 conjugate is an antibody against Src for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: An 1:100 dilution of this antibody from a representative lot detected Src in A431, HUVECs, and NIH/3T3 cells.
      The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications.
      Biological Information
      ImmunogenRecombinant PrA-RSV p60src.
      EpitopeAmino acids 82-169.
      CloneGD11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone GD11 reacted with v-Src of Rous sarcoma virus (RSV) strains Prague subgroup A (PrA-RSV), Schmidt-Ruppin subgroup D (SRD-RSV), and Bratislava 77 subgroup C (B77 C-RSV). Clone GD11 detected enzymatically active c-Src from a variety of chicken, rodent, and human cells (Parsons, S.J., et al. (1984). J. Virol. 51(2):272-82).
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Rat
      • Mouse
      • Human
      • Mink
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • V-SRC
      • SRC
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~60 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: An 1:100 dilution of this antibody detected Src in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications