05-1100X | Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2

30 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, IP, ELISAMPurifiedMonoclonal Antibody
      Catalogue Number05-1100X
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2
      Background InformationSulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasite Plasmodium vivax.
      This clone was derived from the antibody described in Kehoe et al., 2006.
      Product Information
      Presentation100 μl in 100 mM Tris, 150 mM NaCl, 180 mM Glycine, pH 7.5 containing 0.05% sodium azide.
      ApplicationAnti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2 is a high quality Mouse Monoclonal Antibody for the detection of Sulfotyrosine & has been validated in WB, IP, ELISA.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • ELISA
      Application NotesWB, IP, ELISA
      Biological Information
      ImmunogenThis antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC
      EpitopeSulfated Tyrosine
      SpecificityRecognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.
      Species Reactivity
      • All
      Species Reactivity NoteBroad species reactivity. Tested using human, mouse, bovine, and porcine, but the antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.
      Antibody TypeMonoclonal Antibody
      Purification MethodCulture supernatant, purified by protein G affinity chromatography
      Molecular Weightn/a
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAssayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year from date of receipt when stored at +2-8°C.
      Packaging Information
      Material Size30 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 3082472
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2392292 2392292
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2251781 2251781
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - DAM1483552 DAM1483552
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 -2521445 2521445
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 -2586424 2586424
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 -2594842 2594842
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 -2620283 2620283
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 -2716664 2716664
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 -2736598 2736598