05-434 | Anti-Syk Antibody, clone 4D10.1

05-434
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, Enzyme Assays, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-434
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Syk Antibody, clone 4D10.1
      Alternate Names
      • Spleen tyrosine kinase
      Background InformationSyk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration.
      SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.
      References
      Product Information
      FormatPurified
      Control
      • Raji cell lysate
      PresentationProtein A purified IgG2aκ in 10 mM PBS, pH 7.4
      Applications
      ApplicationDetect Syk using this Anti-Syk Antibody, clone 4D10.1 validated for use in IP, EA & WB.
      Key Applications
      • Immunoprecipitation
      • Enzyme Assays
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues 313-339 of human Syk conjugated to KLH.
      Clone4D10.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Syk.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SYK
      Non-Reactive Species
      • Mouse
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43405 # Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion (By similarity).
      SIZE: 635 amino acids; 72066 Da
      SUBUNIT: Interacts with CBL and SLA when it is phosphorylated. The interaction with SLA may link it to CBL, leading to its destruction. Interacts with phosphorylated NFAM1 (By similarity). Interacts with Epstein-Barr virus LMP2A. Interacts through its SH2 domains with the phosphorylated ITAM domain of CD79A which stimulates SYK autophosphorylation and activation.
      PTM: Autophosphorylated. & Phosphorylation on Tyr-323 creates a binding site for c-Cbl, an adapter protein that serves as a negative regulator of BCR- stimulated calcium ion signaling (By similarity). & Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3- kinase-independent pathway (By similarity). & Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation (By similarity).
      SIMILARITY: SwissProt: P43405 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
      Molecular Weight72 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from a Raji cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Syk, clone 4D10.1 2483193
      Anti-Syk, clone 4D10.1 (mouse monoclonal IgG2aκ) - 2453106 2453106
      Anti-Syk, clone 4D10.1 (mouse monoclonal IgG2ak) 2918371
      Anti-Syk, clone 4D10.1 (mouse monoclonal IgG2ak) - 2349579 2349579
      Anti-Syk, clone 4D10.1 - 0609040166 0609040166
      Anti-Syk, clone 4D10.1 - 0702052451 0702052451
      Anti-Syk, clone 4D10.1 - 16094 16094
      Anti-Syk, clone 4D10.1 - 18877 18877
      Anti-Syk, clone 4D10.1 - 19336 19336
      Anti-Syk, clone 4D10.1 - 21859 21859

      References

      Reference overviewApplicationPub Med ID
      An ITAM in a nonenveloped virus regulates activation of NF-κB, induction of beta interferon, and viral spread.
      Stebbing, RE; Irvin, SC; Rivera-Serrano, EE; Boehme, KW; Ikizler, M; Yoder, JA; Dermody, TS; Sherry, B
      Journal of virology  88  2572-83  2014

      Show Abstract
      Western Blotting24352448 24352448
      Differential requirement of ZAP-70 for CD2-mediated activation pathways of mature human T cells.
      E Meinl, D Lengenfelder, N Blank, R Pirzer, L Barata, C Hivroz, E Meinl, D Lengenfelder, N Blank, R Pirzer, L Barata, C Hivroz
      Journal of immunology (Baltimore, Md. : 1950)  165  3578-83  2000

      Show Abstract
      11034358 11034358
      The Syk protein tyrosine kinase can function independently of CD45 or Lck in T cell antigen receptor signaling
      Chu, D H, et al
      EMBO J, 15:6251-61 (1996)  1996

      8947048 8947048
      Syk tyrosine kinase required for mouse viability and B-cell development.
      Cheng, A M, et al.
      Nature, 378: 303-6 (1995)  1995

      Show Abstract
      7477353 7477353