04-1050 | Anti-γ-Synuclein Antibody, clone EP1539Y, rabbit monoclonal

04-1050
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, ICC, FC, IP, IH(P)RbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1050
      DescriptionAnti-γ-Synuclein Antibody, clone EP1539Y, rabbit monoclonal
      Alternate Names
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor
      • alpha-synuclein, isoform NACP140
      • non A-beta component of AD amyloid
      • synuclein, alpha (non A4 component of amyloid precursor)
      Background InformationThe synucleins (there is an alpha, beta, and gamma isoform) are small proteins, less than 140 amino acids, which may function in mictotubule assembly and organization. Synucleins with nitrated tyrosine residues are found in the hallmark lesions of Parkinson's and Lewy Body disease. It has been proposed that the formation of aggregates of nitrated proteins may be at least partly responsible for the cytoxicity of these neurodegenerative diseases.
      References
      Product Information
      FormatUnpurified
      Control
      • Fetal brain lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-γ-Synuclein Antibody, clone EP1539Y, Rabbit is validated for use in WB, IC, FC, IP, IH(P) for the detection of γ-Synuclein.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected β-Tubulin in human brain tissue.
      Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.
      Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.
      Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues on γ-Synuclein.
      CloneEP1539Y
      HostRabbit
      SpecificityThis antibody recognizes γ-Synuclein.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development.
      Gene Symbol
      • MGC110988
      • NACP
      • OTTHUMP00000161559
      • OTTHUMP00000161561
      • PARK1
      • PARK4
      • PD1
      • alpha-synuclein
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
      SUBUNIT STRUCTURE: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1 By similarity. Interacts with phospholipase D and histones.
      SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note: Membrane-bound in dopaminergic neurons. Also found in the nucleus.
      TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
      DOMAIN: The NAC domain is involved in the fibril formation. The middle region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
      PTM: Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form By similarity.
      INVOLVEMENT IN DISEASE: Defects in SNCA are a cause of autosomal dominant Parkinson disease 1 (PARK1) [MIM:168601, 168600]. Parkinson disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years, although early-onset cases (before 50 years) are known. PD generally arises as a sporadic condition but is occasionally inherited as a simple mendelian trait. Although sporadic and familial PD are very similar, inherited forms of the disease usually begin at earlier ages and are associated with atypical clinical features. PD is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
      Defects in SNCA are the cause of Parkinson disease 4 (PARK4) [MIM:605543, 168600].
      Defects in SNCA are the cause of Lewy body dementia (DLB) [MIM:127750]. DLB is a neurodegenerative disorder clinically characterized by dementia and parkinsonism, often with fluctuating cognitive function, visual hallucinations, falls, syncopal episodes, and sensitivity to neuroleptic medication. Presence of Lewy bodies are the only essential pathologic features.
      Deposition of fibrillar amyloid proteins intraneuronally as neurofibrillary tangles is characteristic of Alzheimer disease (AD). SNCA is a minor protein found within these deposits, but a major non amyloid component.
      Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCA is found in LB-like inclusions, glial inclusions and spheroids.
      SEQUENCE SIMILARITIES: Belongs to the synuclein family.
      Molecular Weight~16 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on fetal brain cell lysates.
      Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect γ-Synuclein in fetal brain cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies