04-856 | Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal

04-856
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Ca, ChWB, IPRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-856
      Replaces05-856
      DescriptionAnti-TBK1 Antibody, clone AOW9, rabbit monoclonal
      Alternate Names
      • NF-kB-activating kinase
      • NF-kappa-B-activating kinase
      • TANK-binding kinase 1
      Background InformationTBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • RIPA lysates from HEK293 cells.
      PresentationCultured supernatant containing 0.05% sodium azide.
      Applications
      ApplicationDetect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation:
      1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.
      Biological Information
      ImmunogenPeptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.
      Epitopea.a. 642-645
      CloneAOW9
      HostRabbit
      SpecificityTBK1; Does not cross-react with IKKε, IKKα or IKKβ.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Canine
      • Chicken
      Species Reactivity NoteHuman. Predicted to cross-react with mouse, dog, and chicken based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. [provided by RefSeq]
      Gene Symbol
      • EC 2.7.11.1
      • FLJ11330
      • NAK
      • T2K
      UniProt Number
      UniProt SummaryFUNCTION: Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively. Ref.1 Ref.2 Ref.5 Ref.7 Ref.8 Ref.11

      Catalytic activity ATP + a protein = ADP + a phosphoprotein.

      SUBUNIT: Interacts with TIRAP, TANK and TRAF2. Part of a ternary complex consisting of TANK, TRAF2 and TBK1. Interacts with AZI2. Interacts with SIKE. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between TBK1 and SIKE. Interacts with HCV NS3, a hepatitis C virus protein and with BDV P protein, a Borna disease virus protein. Ref.1 Ref.6 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14

      SUBCELLULAR LOCATION:Cytoplasm. Ref.11

      SPECIFICITY:Ubiquitous with higher expression in testis. Ref.2

      SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

      Contains 1 protein kinase domain.

      Molecular Weight85 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from HEK293 cells.

      Western Blot Analysis:
      A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-TBK1, clone AOW9 2882715
      Anti-TBK1, clone AOW9 - 2211945 2211945
      Anti-TBK1, clone AOW9 - 2561064 2561064
      Anti-TBK1, clone AOW9 - NG1664793 NG1664793
      Anti-TBK1, clone AOW9 - NG1752499 NG1752499
      Anti-TBK1, clone AOW9 - NG1809711 NG1809711
      Anti-TBK1, clone AOW9 - NG1852108 NG1852108
      Anti-TBK1, clone AOW9 - NG1895102 NG1895102
      Anti-TBK1, clone AOW9 -2594360 2594360
      Anti-TBK1, clone AOW9 -2793453 2793453

      References

      Reference overviewPub Med ID
      IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.
      Fitzgerald, Katherine A, et al.
      Nat. Immunol., 4: 491-6 (2003)  2003

      Show Abstract
      12692549 12692549
      Triggering the interferon antiviral response through an IKK-related pathway.
      Sharma, Sonia, et al.
      Science, 300: 1148-51 (2003)  2003

      Show Abstract
      12702806 12702806