05-511 | Anti-TCF-4 Antibody, clone 6H5-3

05-511
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MEMSA, ICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-511
      Replaces04-1080
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-TCF-4 Antibody, clone 6H5-3
      Alternate Names
      • HMG box transcription factor 4
      • Transcription factor 7-like 2
      • T-Cell factor 4, T-cell-specific, HMG box
      • T-Cell-specific transcription factor 4
      • Transcription factor 7-like 2
      Background InformationTCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
      References
      Product Information
      FormatPurified
      Control
      • DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
      PresentationPurified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry/Immunohistochemistry:
      A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).

      Immunoprecipitation:
      A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001).

      Supershift Assay:
      A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).
      Biological Information
      ImmunogenAmino acids 31-331 of human TCF-4.
      Clone6H5-3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes TCF-4
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySOX-TCF_HMG-box, class I member of the HMG-box superfamily of DNA-binding proteins. These proteins contain a single HMG box, and bind the minor groove of DNA in a highly sequence-specific manner. Members include SRY and its homologs in insects and vertebrates, and transcription factor-like proteins, TCF-1, -3, -4, and LEF-1. They appear to bind the minor groove of the A/T C A A A G/C-motif.
      Gene Symbol
      • TCF4
      • TCF-4
      • TCF7L2
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 (By similarity). Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
      SIZE: 619 amino acids; 67919 Da
      SUBUNIT: Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300 (By similarity).
      SUBCELLULAR LOCATION: Nucleus. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies (By similarity).
      TISSUE SPECIFICITY: Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
      DISEASE: Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).
      SIMILARITY: Contains 1 HMG box DNA-binding domain.
      Molecular WeightMr 66 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-TCF-4, clone 6H5-3 (mouse monoclonal IgG2a) - JBC1381947 JBC1381947
      Anti-TCF-4, clone 6H5-3 - 2040540A 2040540A
      Anti-TCF-4, clone 6H5-3 - 2117171 2117171
      Anti-TCF-4, clone 6H5-3 - 2391103 2391103
      Anti-TCF-4, clone 6H5-3 - 0702052450 0702052450
      Anti-TCF-4, clone 6H5-3 - 18791 18791
      Anti-TCF-4, clone 6H5-3 - 1970373 1970373
      Anti-TCF-4, clone 6H5-3 - 2016026 2016026
      Anti-TCF-4, clone 6H5-3 - 2040540 2040540
      Anti-TCF-4, clone 6H5-3 - 20884 20884

      References

      Reference overviewApplicationPub Med ID
      TCF4 Is a Molecular Target of Resveratrol in the Prevention of Colorectal Cancer.
      Jeong, JB; Lee, J; Lee, SH
      International journal of molecular sciences  16  10411-25  2015

      Show Abstract
      25961950 25961950
      Parathyroid hormone-related protein inhibits DKK1 expression through c-Jun-mediated inhibition of β-catenin activation of the DKK1 promoter in prostate cancer.
      Zhang, H; Yu, C; Dai, J; Keller, JM; Hua, A; Sottnik, JL; Shelley, G; Hall, CL; Park, SI; Yao, Z; Zhang, J; McCauley, LK; Keller, ET
      Oncogene  33  2464-77  2014

      Show Abstract
      Chromatin Immunoprecipitation (ChIP)23752183 23752183
      Two novel type 2 diabetes loci revealed through integration of TCF7L2 DNA occupancy and SNP association data.
      Johnson, ME; Zhao, J; Schug, J; Deliard, S; Xia, Q; Guy, VC; Sainz, J; Kaestner, KH; Wells, AD; Grant, SF
      BMJ open diabetes research & care  2  e000052  2014

      Show Abstract
      25469308 25469308
      Genome-wide ChIP-seq analysis of TCF4 binding regions in colorectal cancer cells.
      Chen, C; Lu, Y; Liu, J; Li, L; Zhao, N; Lin, B
      International journal of clinical and experimental medicine  7  4253-9  2014

      Show Abstract
      25550940 25550940
      Nuclear AXIN2 represses MYC gene expression.
      Rennoll, SA; Konsavage, WM; Yochum, GS
      Biochemical and biophysical research communications  443  217-22  2014

      Show Abstract
      Western Blotting24299953 24299953
      The myc 3' wnt-responsive element suppresses colonic tumorigenesis.
      Konsavage, WM; Yochum, GS
      Molecular and cellular biology  34  1659-69  2014

      Show Abstract
      24567369 24567369
      HIV's Nef interacts with β-catenin of the Wnt signaling pathway in HEK293 cells.
      Weiser, K; Barton, M; Gershoony, D; Dasgupta, R; Cardozo, T
      PloS one  8  e77865  2013

      Show Abstract
      Western Blotting24130899 24130899
      Review of the neuroanatomic landscape implicated in glucose sensing and regulation of nutrient signaling: immunophenotypic localization of diabetes gene Tcf7l2 in the developing murine brain.
      Weaver, C; Turner, N; Hall, J
      Journal of chemical neuroanatomy  45  1-17  2012

      Show Abstract
      Immunohistochemistry22796301 22796301
      Human SMC2 protein, a core subunit of human condensin complex, is a novel transcriptional target of the WNT signaling pathway and a new therapeutic target.
      Dávalos, V; Súarez-López, L; Castaño, J; Messent, A; Abasolo, I; Fernandez, Y; Guerra-Moreno, A; Espín, E; Armengol, M; Musulen, E; Ariza, A; Sayós, J; Arango, D; Schwartz, S
      The Journal of biological chemistry  287  43472-81  2012

      Show Abstract
      Western Blotting23095742 23095742
      Regulation of amphiregulin gene expression by β-catenin signaling in human hepatocellular carcinoma cells: a novel crosstalk between FGF19 and the EGFR system.
      Latasa, MU; Salis, F; Urtasun, R; Garcia-Irigoyen, O; Elizalde, M; Uriarte, I; Santamaria, M; Feo, F; Pascale, RM; Prieto, J; Berasain, C; Avila, MA
      PloS one  7  e52711  2012

      Show Abstract
      23285165 23285165

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies