06-1035 | Anti-TMEM199 Antibody

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number06-1035
      DescriptionAnti-TMEM199 Antibody
      Alternate Names
      • transmembrane protein 199
      • chromosome 17 open reading frame 3
      Background InformationTransmembrane protein 199 (TMEM199) is thought to be a multi-pass membrane protein that is part of a collection of proteins sometimes called a NET's (nuclear envelope transmembrane protein). With a predicted mass of ~23 kDa, this nuclear envelope protein was identified using nuclear envelopes from resting and phytohemagglutinin-activated leukocytes. As a result of this activation, these leukocytes have a particularly high density of peripheral chromatin that undergoes significant reorganization.
      Product Information
      FormatAffinity Purified
      • Jurkat cell lysate
      PresentationPurified rabbit polyclonal in PBS containing 0.09% sodium azide.
      ApplicationAnti-TMEM199 Antibody is a Rabbit Polyclonal Antibody for detection of TMEM199 also known as transmembrane protein 199 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot was used by an indpendent laboratory in C2C12 cells (Korfali, N., et al. (2010).
      Biological Information
      ImmunogenLinear peptide corresponding to human TMEM199.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • C17orf32
      • TMEM199
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummarySUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
      Molecular Weight ~19 kDa observed. Weak uncharacterized bands at ~135 and 165 kDa have been observed in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected TMEM199 on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-TMEM199 - 2444684 2444684
      Anti-TMEM199 - 2450107 2450107
      Anti-TMEM199 - NRG1855200 NRG1855200
      Anti-TMEM199 -2634215 2634215


      Reference overviewApplicationPub Med ID
      The leukocyte nuclear envelope proteome varies with cell activation and contains novel transmembrane proteins that affect genome architecture.
      Korfali, N; Wilkie, GS; Swanson, SK; Srsen, V; Batrakou, DG; Fairley, EA; Malik, P; Zuleger, N; Goncharevich, A; de Las Heras, J; Kelly, DA; Kerr, AR; Florens, L; Schirmer, EC
      Molecular & cellular proteomics : MCP  9  2571-85  2010

      Show Abstract
      Western Blotting20693407 20693407
      pH regulatory Na/H exchange by Amphiuma red blood cells.
      P M Cala,H M Maldonado
      The Journal of general physiology  103  1994

      Show Abstract Full Text Article
      7931136 7931136