07-2160 Anti-TRB-3 Antibody

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number07-2160
      DescriptionAnti-TRB-3 Antibody
      Alternate Names
      • tribbles homolog 3 (Drosophila)
      • chromosome 20 open reading frame 97
      • Neuronal cell death-inducible putative kinase
      • p65-interacting inhibitor of NF-kappa-B
      • tribbles homolog 3
      • p65-interacting inhibitor of NF-kappaB
      • neuronal cell death inducible putative kinase
      Background InformationTribbles homolog 3 (TRB-3) is also known as SINK and is thought to play a role in a negative-feedback regulation pathway of NF-kappaB-inducible expression. TRB-3 seems to regulate the level and preciseness of MAPK kinase activation of MAPK. TRB-3 has been found to decrease cell-cycle progression and expression is up-regulated in response to stress. TRB-3 works by blocking Akt activation which inhibits insulin signaling and may play a role in the development of non-alcoholic fatty liver disease (NAFLD).
      Product Information
      FormatAffinity Purified
      • HT-29 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis TRB-3 antibody is validated for use in WB for the detection of the TRB-3 protein.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human TRB-3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes TRB-3 corresponding to the N-terminus.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with orangutan based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. [provided by RefSeq].
      Gene Symbol
      • TRB3
      • TRB-3
      • TRIB3
      • SKIP3
      • NIPK
      • SINK
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.

      SUBUNIT STRUCTURE: Interacts with AKT1, AKT2, ATF4, MAP2K1 and MAP2K7.

      SUBCELLULAR LOCATION: Nucleus (Probable).

      TISSUE SPECIFICITY: Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.

      INDUCTION: By hypoxia, TNF and by nutrient starvation. Expression is PI 3-kinase and/or NF-kappa-B-dependent.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily. Contains 1 protein kinase domain.

      CAUTION: The role of this protein in Akt activation has been demonstrated by Du et al (PubMed:12791994) for the mouse ortholog but Iynedjian (PubMed:15469416) has not been able to reproduce the result in rat hepatocytes.
      Molecular Weight~42 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HT-29 cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected TRB-3 on 10 µg of HT-29 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information