05-1144 | Anti-Talin Antibody, CT, clone TD77

05-1144
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ch, MWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1144
      ReplacesMAB3264
      DescriptionAnti-Talin Antibody, CT, clone TD77
      Alternate Names
      • talin 1
      Background InformationTalin consisting of a 47-kDa N-terminal head domain (residues 1-433) and a 190-kDa
      C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate.
      PresentationPurified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PBS pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.
      Applications
      ApplicationDetect Talin using this Anti-Talin Antibody, C-terminus, clone TD77 validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution of this antibody detected Talin in human GI track tissue.
      Biological Information
      ImmunogenHuman platelet protein
      EpitopeC-terminus
      CloneTD77
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Human Talin, and cross reacts with Mouse and chicken Talin. The epitope recognized by this antibody is located within the C-terminal region of the protein (residues 2269-2541), which also contains an F-actin binding site homologous to that in the yeast actin-binding protein SIa2p.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Chicken
      • Mouse
      Species Reactivity NoteProven to react with human. Expected cross-reactivity in mouse and chicken.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ILWEQ
      • KIAA1027
      • TLN
      Purification MethodPurified from Protein A Sepharose™ chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts By similarity.
      SUBUNIT STRUCTURE: Binds with high affinity to vinculin and with low affinity to integrins. Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM.
      SUBCELLULAR LOCATION: Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with LAYN at the membrane ruffles By similarity.
      SEQUENCE SIMILARITIES: Contains 1 FERM domain.
      Contains 1 I/LWEQ domain.
      Molecular Weight270 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysates.
      Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain between 2 and 8°C for 1 year after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Talin, C-terminus, clone TD77 - 2108575 2108575
      Anti-Talin, C-terminus, clone TD77 - 2148292 2148292
      Anti-Talin, C-terminus, clone TD77 - 2391100 2391100
      Anti-Talin, C-terminus, clone TD77 - 2010692 2010692
      Anti-Talin, C-terminus, clone TD77 - NG1750132 NG1750132
      Anti-Talin, C-terminus, clone TD77 - NG1820245 NG1820245
      Anti-Talin, C-terminus, clone TD77 - NG1916168 NG1916168
      Anti-Talin, C-terminus, clone TD77 - NRG1701184 NRG1701184
      Anti-Talin, C-terminus, clone TD77 -2517791 2517791
      Anti-Talin, C-terminus, clone TD77 -2521447 2521447

      References

      Reference overviewPub Med ID
      Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.
      Wortmann, A; He, Y; Christensen, ME; Linn, M; Lumley, JW; Pollock, PM; Waterhouse, NJ; Hooper, JD
      The Journal of biological chemistry  286  42303-15  2011

      Show Abstract
      21994943 21994943