06-1328 | Anti-Themis Antibody

06-1328
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, FC, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1328
      DescriptionAnti-Themis Antibody
      Alternate Names
      • thymocyte selection associated
      • thymocyte selection pathway associated
      • chromosome 6 open reading frame 190
      • chromosome 6 open reading frame 207
      • protein THEMIS
      • Thymocyte-expressed molecule involved in selection
      Background InformationThymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse thymocyte tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect Themis using this Anti-Themis Antibody validated for use in WB, FC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesWestern Blot (Snap i.d.) Analysis: 2 µg/mL from a previous lot detected Themis in 10 µg of mouse thymocyte tissue lysate.

      Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Vasily Rybakin, PhD., Gascoigne lab, Scripps Research Institute, La Jolla, CA

      Western Blot Analysis: A previous lot of this antibody was used to detect the presence and absence of Themis in Themis KO or WT thymocytes (Fu, G., et al. (2013) Nature. doi: 10.1038/nature12718)

      Immunopreciptiation Analysis: A previous lot of this antibody was used to immunoprecipitate Themis in WT thymocytes but not in Themis KO thymocytes (Fu, G., et al. (2013) Nature. doi: 10.1038/nature12718)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Themis at the C-terminus.
      Species Reactivity
      • Mouse
      Species Reactivity NoteDemonstrated to react with mouse.
      Rat (73% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Themis
      • TSEPA
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.

      SUBUNIT STRUCTURE: Interacts with PLCG1, ITK and GRB2.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus

      TISSUE SPECIFICTY: Expressed in the thymus and to a lesser extent in the spleen but not detectable in nonlymphoid tissues. Highly expressed in thymocytes between the pre-T-cell antigen receptor (pre-TCR) and positive-selection checkpoints and expressed at low level in mature T-cells (at protein level).

      INDUCTION: Down-regulated by stimulation through the alpha-beta TCR.

      PTM: Phosphorylated on Tyr residues quickly after TCR stimulation.

      DISRUPTION: phenotype Mice are viable, are born at the expected mendelian frequency and present no gross abnormalities. They however display a blockade in late T-cell development with defects in thymocyte selection. The number of transitional CD4+CD8(int) thymocytes as well as CD4+ or CD8+ single-positive thymocytes is lower. Thymocytes show defective positive selection, resulting in fewer mature thymocytes. Negative selection is also impaired. A greater percentage of T-cells have CD4+CD25+Foxp3+ regulatory and CD62L(lo)CD44(hi) memory phenotypes compared to wild-type T-cells.

      SEQUENCE SIMILARITIES: Belongs to the themis family.
      Molecular Weight~72 kDa observed.
      UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse thymocyte tissue lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Themis ANTI-THEMIS
      Anti-Themis 2475900
      Anti-Themis - 2140084 2140084
      Anti-Themis - 2382226 2382226
      Anti-Themis - 2386527 2386527
      Anti-Themis - 1990440 1990440
      Anti-Themis - 2107265 2107265
      Anti-Themis - 2224486 2224486
      Anti-Themis - 2329168 2329168
      Anti-Themis - 2358870 2358870

      References

      Reference overviewApplicationPub Med ID
      Differential function of Themis CABIT domains during T cell development.
      Okada, T; Nitta, T; Kaji, K; Takashima, A; Oda, H; Tamehiro, N; Goto, M; Okamura, T; Patrick, MS; Suzuki, H
      PloS one  9  e89115  2014

      Show Abstract
      24586531 24586531
      Themis sets the signal threshold for positive and negative selection in T-cell development.
      Fu, G; Casas, J; Rigaud, S; Rybakin, V; Lambolez, F; Brzostek, J; Hoerter, JA; Paster, W; Acuto, O; Cheroutre, H; Sauer, K; Gascoigne, NR
      Nature  504  441-5  2013

      Show Abstract
      Immunoprecipitation24226767 24226767