Key Spec Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|M, R||FC, ICC||M||FITC||Monoclonal Antibody|
|Description||Anti-Thy-1 Antibody, clone OX-7, FITC conjugated|
|Presentation||The conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.|
|Material Size||100 assays|
|Reference overview||Pub Med ID|
|Culture of human mesenchymal stem cells using a candidate pharmaceutical grade xeno-free cell culture supplement derived from industrial human plasma pools.|
Díez, JM; Bauman, E; Gajardo, R; Jorquera, JI
Stem cell research & therapy 6 28 2015
Fetal bovine serum (FBS) is an animal product used as a medium supplement. The animal origin of FBS is a concern if cultured stem cells are to be utilized for human cell therapy. Therefore, a substitute for FBS is desirable. In this study, an industrial, xeno-free, pharmaceutical-grade supplement for cell culture (SCC) under development at Grifols was tested for growth of human mesenchymal stem cells (hMSCs), cell characterization, and differentiation capacity.SCC is a freeze-dried product obtained through cold-ethanol fractionation of industrial human plasma pools from healthy donors. Bone marrow-derived hMSC cell lines were obtained from two commercial suppliers. Cell growth was evaluated by culturing hMSCs with commercial media or media supplemented with SCC or FBS. Cell viability and cell yield were assessed with an automated cell counter. Cell surface markers were studied by indirect immunofluorescence assay. Cells were cultured then differentiated into adipocytes, chondrocytes, osteoblasts, and neurons, as assessed by specific staining and microscopy observation.SCC supported the growth of commercial hMSCs. Starting from the same number of seeded cells in two consecutive passages of culture with medium supplemented with SCC, hMSC yield and cell population doubling time were equivalent to the values obtained with the commercial medium and was consistent among lots. The viability of hMSCs was higher than 90%, while maintaining the characteristic phenotype of undifferentiated hMSCs (positive for CD29, CD44, CD90, CD105, CD146, CD166 and Stro-1; negative for CD14 and CD19). Cultured hMSCs maintained the potential for differentiation into adipocytes, chondrocytes, osteoblasts, and neurons.The tested human plasma-derived SCC sustains the adequate growth of hMSCs, while preserving their differentiation capacity. SCC can be a potential candidate for cell culture supplement in advanced cell therapies.
|Do mesenchymal stem cells modulate the milieu of reconstructed bladder wall?|
Pokrywczynska, M; Jundzill, A; Bodnar, M; Adamowicz, J; Tworkiewicz, J; Szylberg, L; Debski, R; Marszalek, A; Drewa, T
Archivum immunologiae et therapiae experimentalis 61 483-93 2013
To evaluate the mesenchymal stem cells (MSCs) influence on cytokines and matrix metalloproteinases (MMPs) expression in rat bladder wall regeneration. MSCs cultures from the bone marrow were established. Acellular matrices from the bladder submucosa were prepared. Bladders were reconstructed using cell-seeded (n = 5) and unseeded (n = 5) grafts. MSCs were injected into the bladder wall (n = 5), bladders were incised and MSCs were injected into the circulation (n = 5) or were left intact (n = 5). Animals were killed after 3 months. Bladder histology and immunohistochemical staining of IL-2, IL-4, IL-6, IL-10, TNF-α, TGF-β1, IFN-γ, MMP-2, and MMP-9 were done. Bladders reconstructed with cell-seeded grafts mimicked native tissue, while unseeded grafts revealed shrinkage and morphological irregularities. There were no morphological changes in bladders of other groups. Different pattern of cytokine and MMP expression was observed. Increased expression of anti-inflammatory cytokines and MMPs in bladder promotes detrusor regeneration.
|Monoclonal antibody-ricin or ricin A chain hybrids: kinetic analysis of cell killing for tumor therapy.|
Neville, D M and Youle, R J
Immunol. Rev., 62: 75-91 (1982) 1982
|Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin.|
Williams, A F and Gagnon, J
Science, 216: 696-703 (1982) 1982
The amino acid sequences of mouse brain Thy-1 glycoproteins are shown to be homologous to those of variable-region immunoglobulin domains. There is also good homology with constant domains and beta 2-microglobulin; overall the results suggest that Thy-1 may be like the primordial immunoglobulin domain. Preliminary evidence for an invertebrate Thy-1 homolog supports this possibility.
|The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.|
Barclay, A N
Immunology, 42: 593-600 (1981) 1981
The localisation of subpopulations of T lymphocytes as defined by the monoclonal antibodies W3/25 and MRC OX 8 was determined by an indirect immunoperoxidase technique on cryostat sections of rat lymphoid tissues. Both subpopulations were present throughout T-dependent areas with only scattered T cells, largely W3/25 positive, in the B areas. The W3/25 antigen was also found on macrophages in the medulla of lymph nodes and in the peritoneal cavity and liver.
|The kinetics of antibody binding to membrane antigens in solution and at the cell surface.|
Mason, D W and Williams, A F
Biochem. J., 187: 1-20 (1980) 1980
The reaction kinetics of 125I-labelled mouse monoclonal antibodies binding to three cell-surface antigens of rat thymocytes (Thy-1.1, W3/25) were studied. The differences between bivalent and univalent interactions were determined by using antibody in the F(ab')2 or Fab' form and by using antigen in polymeric or monomeric forms. Association rate constants (k+1), dissociation rate constants (k-1) and equilibrium constants were determined. Also, the dissociation kinetics of rabbit antibodies against rat Thy-1 antigen were studied. The major findings were as follows. (i) With F(ab')2 antibody there was no simple relationship between antigen density at the cell surface and extent of bivalent binding. Extensive univalent binding was observed unless the antibody had a high k-1 for the univalent interaction, in which case all binding was bivalent. (ii) k+1 values were similar for F(ab')2 or Fab' antibody, and for the different antibodies were in the range 0.8 x 10(5)--1.1 x 10(6) M-1.s-1. These differences were sufficient to affect the interpretation of serological assays with the different antibodies. (iii) Antibody bound bivalently dissociated much more slowly than that bound univalently. However, the k-1 values for the univalently bound antibody were sufficiently low in most cases that the lifetime of the univalent complex was similar to or greater than the time needed for the assay. Thus the results could be interpreted on the basis of irreversible reactions. The overall conclusion from the study is that for an understanding of the binding of antibody to cell-surface antigens the kinetics of the interaction are of major importance and theories based on equilibrium binding are inappropriate.
|Identification of Ia glycoproteins in rat thymus and purification from rat spleen.|
McMaster, W R and Williams, A F
Eur. J. Immunol., 9: 426-33 (1979) 1979
|MOUSE ANTI-RAT THY-1 (CD90.1) FITC CONJUGATED|