07-038 Anti-Tip60 Antibody

07-038
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-038
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Tip60 Antibody
      Alternate Names
      • 60 kDa Tat interactive protein
      • HIV-1 Tat interactive protein
      • HIV-1 Tat interactive protein
      • 60kDa
      • K(lysine) acetyltransferase 5
      • K-acetyltransferase
      • Tat interacting protein, 60kDa
      • cPLA(2)-interacting protein
      • cPLA2 interacting protein
      • Histone acetyltransferase KAT5
      • KAT5
      Background InformationHistone acetyltransferase HTATIP (Tip60). Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Direct binding of HTATIP to the TAT protein of the human immunodeficiency virus (HIV) might be an important feature for efficient TAT transactivation of HIV gene expression.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate and RIPA lysates
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.
      Applications
      ApplicationUse Anti-Tip60 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Tip60 also known as HIV-1 Tat interactive protein, K(lysine) acetyltransferase 5, K-acetyltransferase.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 494-513 (RIDSKCLHF-TPKDWSKRGKW) of human Tip60.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Tip60, Mr 60 kDa. Additional proteins were detected at 66 kDa and 80 kDa in some human and mouse cell lines.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.
      Gene Symbol
      • EC 2.3.1.48
      • EC 2.3.1.-
      • ESA1
      • HTATIP
      • HTATIP1
      • PLIP
      • TIP
      • TIP60
      • Tip60
      • cPLA2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92993 # Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to HTATIP polyubiquitination and targets it to degradation.
      SIZE: 513 amino acids; 58582 Da
      SUBUNIT: Interacts with the cytoplasmic tail of APP (By similarity). Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Interacts with ATM. Interacts with PHF17. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
      PTM: Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity. & Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
      SIMILARITY: SwissProt: Q92993 ## Belongs to the MYST (SAS/MOZ) family.
      Molecular WeightApprox. 60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected Tip60 in RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA.
      Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tip60 - 24645802464580
      Anti-Tip60 - 19626611962661
      Anti-Tip60 - 1990519905
      Anti-Tip60 - 20209162020916
      Anti-Tip60 - 20879332087933
      Anti-Tip60 - 22104032210403
      Anti-Tip60 - 22995722299572
      Anti-Tip60 - 2501225012
      Anti-Tip60 - 2501225012
      Anti-Tip60 - DAM1503359DAM1503359

      References

      Reference overviewApplicationPub Med ID
      Modulation of chromatin remodelling induced by the freshwater cyanotoxin cylindrospermopsin in human intestinal caco-2 cells.
      Huguet, A; Hatton, A; Villot, R; Quenault, H; Blanchard, Y; Fessard, V
      PloS one  9  e99121  2014

      Show Abstract
      24921660 24921660
      Zinc finger protein 668 interacts with Tip60 to promote H2AX acetylation after DNA damage.
      Hu, R; Wang, E; Peng, G; Dai, H; Lin, SY
      Cell cycle (Georgetown, Tex.)  12  2033-41  2013

      Show Abstract
      23777805 23777805
      Characterisation of a Tip60 specific inhibitor, NU9056, in prostate cancer.
      Coffey, K; Blackburn, TJ; Cook, S; Golding, BT; Griffin, RJ; Hardcastle, IR; Hewitt, L; Huberman, K; McNeill, HV; Newell, DR; Roche, C; Ryan-Munden, CA; Watson, A; Robson, CN
      PloS one  7  e45539  2012

      Show Abstract
      23056207 23056207
      The transcriptional specificity of NF-κB dimers is coded within the κB DNA response elements.
      Wang, VY; Huang, W; Asagiri, M; Spann, N; Hoffmann, A; Glass, C; Ghosh, G
      Cell reports  2  824-39  2012

      Show Abstract
      23063365 23063365
      Tip60-mediated acetylation activates transcription independent apoptotic activity of Abl.
      Jiang, Z; Kamath, R; Jin, S; Balasubramani, M; Pandita, TK; Rajasekaran, B
      Molecular cancer  10  88  2011

      Show Abstract
      21781306 21781306
      Perturbation of BRD4 protein function by BRD4-NUT protein abrogates cellular differentiation in NUT midline carcinoma.
      Yan, J; Diaz, J; Jiao, J; Wang, R; You, J
      The Journal of biological chemistry  286  27663-75  2011

      Show Abstract
      21652721 21652721
      Histone H2A.Z is essential for estrogen receptor signaling.
      Nicolas Gévry, Sara Hardy, Pierre-Etienne Jacques, Liette Laflamme, Amy Svotelis, François Robert, Luc Gaudreau, Nicolas Gévry, Sara Hardy, Pierre-Etienne Jacques, Liette Laflamme, Amy Svotelis, François Robert, Luc Gaudreau, Nicolas Gévry, Sara Hardy, Pierre-Etienne Jacques, Liette Laflamme, Amy Svotelis, François Robert, Luc Gaudreau
      Genes development  23  1522-33  2009

      Show Abstract Full Text Article
      19515975 19515975
      Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation.
      Metzger, E; Yin, N; Wissmann, M; Kunowska, N; Fischer, K; Friedrichs, N; Patnaik, D; Higgins, JM; Potier, N; Scheidtmann, KH; Buettner, R; Schüle, R
      Nature cell biology  10  53-60  2008

      Show Abstract Full Text Article
      18066052 18066052
      FOXP3 interactions with histone acetyltransferase and class II histone deacetylases are required for repression.
      Bin Li, Arabinda Samanta, Xiaomin Song, Kathryn T Iacono, Kathryn Bembas, Ran Tao, Samik Basu, James L Riley, Wayne W Hancock, Yuan Shen, Sandra J Saouaf, Mark I Greene, Bin Li, Arabinda Samanta, Xiaomin Song, Kathryn T Iacono, Kathryn Bembas, Ran Tao, Samik Basu, James L Riley, Wayne W Hancock, Yuan Shen, Sandra J Saouaf, Mark I Greene
      Proceedings of the National Academy of Sciences of the United States of America  104  4571-6  2007

      Show Abstract Full Text Article
      17360565 17360565
      Distinct mammalian SWI/SNF chromatin remodeling complexes with opposing roles in cell-cycle control.
      Nagl, NG; Wang, X; Patsialou, A; Van Scoy, M; Moran, E
      EMBO J  26  752-63  2007

      Show Abstract Full Text Article
      17255939 17255939

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