05-1515 | Anti-Transportin 1 Antibody, clone D45

05-1515
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, RbWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1515
      DescriptionAnti-Transportin 1 Antibody, clone D45
      Alternate Names
      • Importin beta-2
      • Karyopherin beta-2
      • M9 region interaction protein
      • importin 2
      • importin beta 2
      • karyopherin (importin) beta 2
      • transportin 1
      Background InformationTransportin 1 (Trn1) is a transport receptor that transports substrates from the cytoplasm to the nucleus through nuclear pore complexes by recognizing nuclear localization signals (NLSs). Transportin 1 is a mediator of hnRNP A1 transport into the nucleus.
      Trn1 has two sites for binding NLSs, one with high affinity and second with low affinity. Interactions at the low affinity site controls overall binding affinity. Trn1 recognizes NLSs at the high affinity site followed by a conformational change at the low affinity site that interacts with NLSs. Alternate splicing of the Trn1 gene results in two transcript variants encoding different proteins.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Transportin 1 Antibody, clone D45 is a Mouse Monoclonal Antibody for detection of Transportin 1 also known as karyopherin (importin) beta 2 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human Transportin-1.
      EpitopeUnknown
      CloneD45
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Transportin-1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Monkey
      • Rabbit
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with monkey and rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq].
      Gene Symbol
      • TNPO1
      • TRN
      • IPO2
      • KPNB2
      • MIP
      • MIP1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity. Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

      SUBUNIT STRUCTURE: Binds HNRPA1, HNRPA2, HNRPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with H2A, H2B, H3 and H4 histones. Binds to HIV-1 Rev.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

      SEQUENCE SIMILARITIES: Belongs to the importin beta family.

      Contains 8 HEAT repeats.

      Contains 1 importin N-terminal domain.
      Molecular Weight~ 102 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected Transportin-1 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Transportin 1, clone D45 - 2266785 2266785
      Anti-Transportin 1, clone D45 - NG1941999 NG1941999
      Anti-Transportin 1, clone D45 - NRG1687959 NRG1687959
      Anti-Transportin 1, clone D45 -2845081 2845081

      References

      Reference overviewApplicationPub Med ID
      Novel somatic single nucleotide variants within the RNA binding protein hnRNP A1 in multiple sclerosis patients.
      Lee, S; Levin, M
      F1000Research  3  132  2014

      Show Abstract
      Immunofluorescence25254102 25254102