05-1339-S | Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304, Trial Size

05-1339-S
10 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, VrtELISA, ICC, IP, WB, Mplex, DBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1339-S
      DescriptionAnti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304, Trial Size
      Alternate Names
      • H3K4me3
      • Histone H3 (trimethyl K4)
      • H3 histone family, member T
      • Histone 3, H3
      • Histone cluster ,
      • H3
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5' end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Acid extract lysate
      PresentationPurified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304, Trial Size is a mouse monoclonal antibody for detection of Trimethyl Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in ELISA, ICC, IP, WB, Mplex, DB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      • Multiplexing
      • Dot Blot
      Application NotesELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA.1

      Immunocytochemistry:
      This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

      Immunoprecipitation:
      This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1

      Multiplexing:
      This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.
      EpitopeTrimethylated Lys4
      CloneCMA304
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • H3.4
      • H3/g
      • H3/t
      • H3FT
      • H3T
      • H3t
      • MGC126886
      • MGC126888
      • OTTHUMP00000037945
      Modifications
      • Methylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Expressed in testicular cells.

      Developmental stage Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM:Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.

      Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.

      Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.

      Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.

      Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.

      Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

      Ubiquitinated By similarity.

      SIMILARITY: Belongs to the histone H3 family.
      Molecular Weightapprox. 17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

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      Life Science Research > Antibodies and Assays > Primary Antibodies