05-1250 | Anti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308

05-1250
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, VrtWB, ELISA, ICC, IP, MplexMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1250
      DescriptionAnti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308
      Alternate Names
      • H3K9me3
      • Histone H3 (tri methyl K9)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Acid extract.
      PresentationPurified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308 is a mouse monoclonal antibody for detection of Trimethyl Histone H3(Lys9) also known as H3K9me3, Histone H3 (tri methyl K9) & has been validated in WB, ELISA, ICC, IP, Mplex.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunocytochemistry
      • Immunoprecipitation
      • Multiplexing
      Application NotesELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA.1

      Immunoprecipitation:
      This antibody has been shown by an outside laboratory to be suitable for IP.1

      Immunocytochemistry:
      This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 1-19 of human Histone H3, trimethylated on Lys9, conjugated to KLH.
      EpitopeTrimethyl Lys9
      CloneCMA308
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is >27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
      Gene Symbol
      • H3.
      • H3/g
      • H3/t
      • H3FT
      • H3T
      • H3t
      • MGC126886
      • MGC126888
      • OTTHUMP00000037945
      Modifications
      • Methylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight~17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      A 0.5 – 2 μg/mL dilution of this antibody detected histone H3 in 10 μg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - 2360596 2360596
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - 2455672 2455672
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - 1943283 1943283
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - 1987493 1987493
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - 2070913 2070913
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - 2205001 2205001
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - NG1713096 NG1713096
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - NG1740981 NG1740981
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - NG1853761 NG1853761
      Anti-trimethyl Histone H3(Lys9), clone CMA308 - NG1922396 NG1922396

      References

      Reference overviewPub Med ID
      The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity.
      Ivanauskiene, K; Delbarre, E; McGhie, JD; Küntziger, T; Wong, LH; Collas, P
      Genome research  24  1584-94  2014

      Show Abstract
      25049225 25049225
      HDA6 directly interacts with DNA methyltransferase MET1 and maintains transposable element silencing in Arabidopsis.
      Liu, X; Yu, CW; Duan, J; Luo, M; Wang, K; Tian, G; Cui, Y; Wu, K
      Plant physiology  158  119-29  2012

      Show Abstract Full Text Article
      21994348 21994348
      The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
      Kimura, Hiroshi, et al.
      Cell Struct. Funct., 33: 61-73 (2008)  2008

      18227620 18227620
      Epigenetic histone H3 lysine 9 methylation in metabolic memory and inflammatory phenotype of vascular smooth muscle cells in diabetes.
      Villeneuve, Louisa M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 105: 9047-52 (2008)  2008

      18579779 18579779
      Differentially expressed genes are marked by histone 3 lysine 9 trimethylation in human cancer cells.
      Wiencke, JK; Zheng, S; Morrison, Z; Yeh, RF
      Oncogene  27  2412-21  2008

      Show Abstract
      17968314 17968314

      Related Products & Applications

      Related Products

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies